摘要：
Objective To study the effects of Puerarin(Pue)on survival rate,expression of GAP-43 and NGF in spinal motoneurons following brachial roots avulsion. Methods From March, 2014 to December, 2015, 192 adult Sprague Dawley rats were divided into four groups: Avulsion, Pue 50 treatment, Pue 100 and Pue 200 groups. The right C5-C7nerve roots were avulsed through the methods of cervical dorsal approach. Puerarin or normal saline was given immediatedly once daily to the rats respectively by the intraperitoneal injection. The rats were killed at 1, 2, 4 and 6 weeks after injury. The paraffin sections of C7 segment were stained with neutral red. Expression of GAP-43 and NGF were detected by Western blot. Results Compared to the avuled group, the motoneuron survival rates of Pue 100 and Pue 200 dose treatment groups increased on the second week and the sixth week(P < 0.05 or P < 0.01), and the Pue 200 dose treatment group increased on the fourth week(P < 0.01).In the anterior horn of all three groups, expression of GAP-43 increased from the 1st week to the 6 week after the operation, and reached the peak at the 4th week, and decreased at the 6th week. Compared to the avuled group, expression of GAP-43 protein increased in the Pue 100 and Pue 200 dose treatment groups at the 1st week and 2nd week,the expression of NGF protein increased in the three Pue treatment groups at four time points(P < 0.05 or P < 0.01). Conclusion Puerarin can improve the survival rates of spinal motoneurons after the brachial plexus root avulsion, and the expression of GAP-43 and NGF increased, suggesting that Puerarin may play an role in the repair of brachial plexus avulsions by promoting the ex-pression of nerve growth factors.%目的 探讨葛根素(Pue)对臂丛根性撕脱后脊髓前角运动神经元成活率、GAP-43及NGF蛋白表达的影响.方法 2014年3月至2015年12月,成年雄性SD大鼠192只,颈、背侧入路撕脱右侧C5~ C7脊神经前根建立臂丛根性撕脱模型.造模后随机分为4组:撕脱组Pue大、中、小剂量治疗组,每组48只,每组又按4个时间(1、2、4、6周)分为4个亚组,每组12只.中性红染色检测脊髓运动神经元的成活率,Western blot检测损伤侧脊髓前角GAP-43及NGF蛋白的表达.结果 在撕脱后第2和第6周,Pue中、大剂量治疗组神经元成活率较撕脱组有显著性增高,在撕脱后第4周,Pue大剂量治疗组神经元成活率较撕脱组显著增高(P < 0.01);GAP-43蛋白表达随撕脱时间的延长而增高,在第4周达到高峰,第6周较低,且在撕脱后第1,2周,葛根素中、高剂量治疗组GAP-43蛋白的表达较撕脱组有显著提高;葛根素低、中、高治疗组NGF蛋白表达量在4个时间点均较模型组显著增高(P<0.05或P<0.01).结论 葛根素可提高臂丛根性撕脱后脊髓脊髓前角运动神经元的成活率,并上调 GAP-43和NGF蛋白的表达,提示葛根素可通过上调 GAP-43和NGF蛋白的表达提高臂丛根性撕脱后脊髓前角运动神经元成活率,在臂丛根性撕脱伤的修复过程中发挥保护作用.
