DNA指纹法

摘要： Objective To explore the bacteria relevance between index fingers and contactant' surfaces (mobile phone touch screen and desktop of personal office table). Methods Bacteria were collected from the index fingers, mobile phone touch screen and desktop of personal office table of 10 volunteers. Enterobacterial repetitive intergenic consensus(ERIC)-PCR fingerprint was established by PCR amplifi-cation technique of metagenome. Results There were 7 volunteers' ERIC-PCR fingerprints of index fin-gers matched that took from the mobile phone touch screens, and different from each other. There were 3 volunteers' ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table, and other 7 volunteers' ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table,but had at least one similar band for both. Conclusion The bacteria on index finger shows individual specificity, which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.%目的 探讨示指菌群和接触物(手机触摸屏、个人办公桌桌面)表面菌群之间的关联性. 方法 采集10名志愿者的示指、手机触摸屏、个人办公桌桌面的菌群,通过宏基因组pCR扩增技术建立肠道细菌基因间重复序列(enterobacterial repetitive intergenic consensus,ERIC)-pCR指纹图谱. 结果7名志愿者的示指与手机触摸屏菌群ERIC-pCR指纹图谱对应吻合,且各志愿者个体之间ERIC-pCR指纹图谱互不相同;3名志愿者的示指菌群与个人办公桌桌面菌群ERIC-pCR指纹图谱对应吻合,其余7名志愿者的示指菌群与个人办公桌桌面菌群ERIC-pCR指纹图谱不完全吻合,但分别存在大小一致的条带.结论 示指所携带的细菌菌群具有一定个体特异性,示指接触手机触摸屏、办公桌桌面后残留下来的菌群,可作为潜在的法医学鉴定的新型生物样本.

摘要： 目的 初步探索尸体肠道菌群的时序性变化用于死亡时间推断.方法 利用棉签擦拭颈椎脱臼处死大鼠肠道取样,通过提取肠道菌群DNA,选取16S rRNA V3区通用引物进行PCR,PCR产物进行变性梯度凝胶电泳,对得到的图谱进行组间菌群多样性与相似性分析,并对从变性梯度凝胶电泳切下的条带进行纯化、PCR、测序后获得各组厚壁菌门的主要菌群所占百分比.结果 处死后大鼠第1～30天的菌群多样性呈现减少趋势(P<0.05),组内相似性呈现下降趋势(P<0.05),第1天指纹图谱条带数、组内相似性系数(similarity coefficient,Cs)高于其他组(P<0.05),第5天与第25、30天组内Cs比较差异有统计学意义(P<0.05).在属水平,处死后第1、5天肠道菌群以肠球菌属(Enterococcus sp.)为主,第10、15、20天以苏云金芽孢杆菌(Bacillus thuringienssis)为主,第25、30天以粪肠球菌(Enterococcus faecalis)为主.结论 肠道菌群随大鼠处死后时间的延续,其组成、结构都出现了明显的变化,肠道菌群的演替与死亡时间相关.

摘要： 目的 利用中药血清药物化学的方法研究十精丸的入血成分.方法 建立口服十精丸后大鼠血清的HPLC指纹图谱,比较十精丸、缺味方、单味药及给药后的血清样品,确定口服十精丸后大鼠血中移行成分及来源生药.结果 十精丸给药后血中出现14个移行成分,4个为血中固有成分,7个为十精丸中原型成分,3个为代谢产物.结论 阐明了十精丸的部分入血移行成分,为进一步研究十精丸的药效物质基础提供参考.%Objective To conduct serum pharmacochemistry study onShijing pill. Methods HPLC fingerprints of serum in rats after takingShijingpill were established,the serun samples after takingShijing pill, decoction without one of the component drugs and single crude drug were compared,the transitional constituents absorbed into the blood and the original crude after takingShijing pill was determined.Results Fourteen transitional constituents were detected in rat blood after takingShijing pill,four of which were permanent component of blood,seven of which were prototype constituents,three of which were metabolites. Conclusions Some of chemical composition ofShijing pill was clarified preliminarily,that contributes to further studying pharmacodynamic meterial foundation ofShijing pill.

摘要： Objective To recognize the possibility of Y fragment deletion of Amelogenin gene intuitively and simply according to the genotyping graphs.Methods By calculating the ratio of total peak height of genotyping graphs, the statistics of equilibrium distribution between Amelogenin and D3S1358loci,Amelo-genin X-gene and Amelogenin Y-gene, and different alleles of D3S1358loci from 1968 individuals was analyzed after amplified by PowerPlex? 21 detection kit.Results Sum of peak height of Amelogenin X allele was not less than 60% that of D3S1358loci alleles in 90.8% female samples, and sum of peak height of Amelogenin X allele was not higher than 70% that of D3S1358loci alleles in 94.9% male samples.Conclusion The result of genotyping after amplified by PowerPlex? 21 detection kit shows that the possibility of Y fragment deletion should be considered when only Amelogenin X-gene of Amelo-geninis detected and the peak height of AmelogeninX-gene is not higher than 70% of the total peak height of D3S1358loci.%目的 依据分型图谱,直观、简便识别是否存在Amelogenin基因Y片段丢失的可能.方法 采用计算分型图谱中等位基因峰高之和比值的方法,统计1968份人员样本经PowerPlex?21试剂盒扩增后Amelogenin与D3S1358基因座之间的均衡性、Amelogenin X等位基因与Amelogenin Y等位基因的均衡性以及D3S1358基因座不同等位基因的均衡性情况.结果 90.8%的女性样本Amelogenin X等位基因峰高不低于D3S1358基因座等位基因峰高和的60%,94.9%的男性样本Amelogenin X等位基因的峰高不超过D3S1358基因座等位基因峰高和的70%.结论 PowerPlex?21试剂盒扩增后的分型结果中,当Amelogenin基因只检测到Amelogenin X等位基因,且Amelogenin X等位基因的峰高不及D3S1358基因座等位基因峰高之和的70%时,应考虑存在Y片段丢失的可能性.

摘要： Objective To explore the fingerprint characteristic peaks contribute to xanthine oxidase inhibitory activity of ethyl acetate extracts from medicinal and edible Chinese materia medica in Citrus,and to characterize the material basis.Methods The HPLC fingerprints of ethyl acetate extracts from Aurantii Fructus Immaturus,Cirri Reticulatae Pericarpium Viride,Cirri Reticulatae Pericarpium,Cirri Exocarpium Rubrum,and Citri Fructus were established,and the degree of enzyme inhibition was calculated by measuring the formation amount of uric acid in the xanthine oxidase-xanthine reaction system.The spectrum-effect relationship was analyzed by grey relation analysis.The mainly characteristic peaks were identified by comparision with reference standards.Results The xanthine oxidase inhibitory activity was resulted from the combined action of multiple components.The contribution order was as follows:eriocitrin,naringin,naringenin,hesperidin,tangeretin,nobiletin,neohesperidin,and hesperetin,respectively.Conclusion The relationships between the fingerprint characteristic peaks of ethyl acetate extracts from Chinese materia medica in Citrus and xanthine oxidase inhibitory activity are paralleled to some extent.%目的 阐明不同柑橘属药材乙酸乙酯提取物指纹图谱特征峰对黄嘌呤氧化酶抑制作用的贡献程度.方法 建立枳实、青皮、陈皮、橘红和香橼乙酸乙酯提取物指纹图谱,HPLC法测定黄嘌呤氧化酶-黄嘌呤反应体系尿酸生成量,评价各提取物对黄嘌呤氧化酶活性的影响,采用灰关联度分析法研究谱效关系,对照品比对法鉴定主要色谱峰.结果 谱效关系分析表明,各药材提取物的黄嘌呤氧化酶抑制活性是多成分共同作用的结果,各色谱峰所代表的化学成分对抑制黄嘌呤氧化酶活性的贡献大小顺序为圣草次苷＞柚皮苷＞柚皮素＞橙皮苷＞桔皮素＞川陈皮素＞新橙皮苷＞橙皮素.结论 不同柑橘属药材乙酸乙酯提取物指纹图谱特征峰与黄嘌呤氧化酶抑制作用有一定的对应关系.

摘要： 目的：建立金精固体饮料的HPLC指纹图谱，为有效评价其质量提供参考。方法采用HPLC法对10批金精固体饮料样品进行测定，以Kromasil C18(4.6 mm×250 mm，5μm)为色谱柱，流动相为乙腈-0.1%磷酸溶液，梯度洗脱，检测波长327 nm，流速1.0 ml/min，柱温35°C。采用中药色谱指纹图谱相似度评价软件对结果进行评价。结果建立了金精固体饮料的 HPLC 指纹图谱，并标定了包括绿原酸在内的12个共有峰，10批样品的相似度均＞0.9。结论该法操作简便可靠，所建立的指纹图谱重现性良好，可作为控制和评价金精固体饮料质量的方法。%Objective To establish an HPLC fingerprint ofJinjing solid beverage,and provide experimental evidence of evaluating its quality.Methods Ten batches ofJinjing solid beverage were analyzed by HPLC under the gradient elution condition. A Kromasil C18 column(4.6 mm×250 mm, 5μm) was used, and the flow phase was acetonitrile-H2O(acidified to 0.1% with phosphoric acid) with gradient elution, and the detection wavelength was 327 nm, and the flow rate was 1.0 ml/min, and the column temperature was 35°C. The data were evaluated by the similarity evaluation software for TCM fingerprint.Results The HPLC fingerprint ofJinjing solid beverage were established. Twelve common peaks including chlorogenic acid were identified with similarity of more than 0.9.Conclusion HPLC method is a reliable, available and quick method, that provides a means for controlling and evaluating the quality ofJinjing solid beverage.

摘要： 目的：评价导流杂交基因芯片技术在女性生殖道人乳头状瘤病毒（ HPV）感染检测中的应用价值,探讨女性生殖道感染最常见的HPV基因型及好发年龄。方法采用导流杂交基因芯片技术对545例患者的宫颈细胞学标本进行宫颈细胞21种HPV-DNA基因分型检测。比较细胞学正常组（n=120）、细胞学异常组（n=425）及细胞学异常各类型[宫颈慢性炎症174例、宫颈上皮内瘤变（ CIN）Ⅰ级102例、CIN Ⅱ级74例、CIN Ⅲ级36例、宫颈癌16例和尖锐湿疣23例]组HPV阳性率。将细胞学异常组分为4个年龄组：20~岁组85例、31~岁组140例、41~岁组90例、51~岁组110例,比较各年龄组HPV阳性率。结果细胞学正常组HPV阳性率为22.5%（27/120）,细胞学异常组为87.3%（371/425）。细胞学异常组及细胞学异常类型6组HPV阳性率分别与细胞学正常组比较,差异均有统计学意义（P〈0．01）。细胞学正常组中6种最为常见的HPV基因型按阳性率递减依次为HPV16、68、18、52、58、11；细胞学异常组中6种最常见的HPV基因型依次为HPV 16、52、58、18、33、31。31~岁组、41~岁组HPV阳性率显著高于51~岁组（P〈0．01）,31~岁组HPV阳性率最高,41岁后随年龄的增长逐渐降低。结论采用导流杂交基因芯片技术一次试验能联合检测出多种HPV亚型感染及型别分布,从而提高由HPV感染引起的妇科肿瘤的防治水平。

摘要： 目的：评价导流杂交基因芯片技术在女性生殖道人乳头状瘤病毒( HPV)感染检测中的应用价值,探讨女性生殖道感染最常见的HPV基因型及好发年龄。方法采用导流杂交基因芯片技术对545例患者的宫颈细胞学标本进行宫颈细胞21种HPV-DNA基因分型检测。比较细胞学正常组(n=120)、细胞学异常组(n=425)及细胞学异常各类型[宫颈慢性炎症174例、宫颈上皮内瘤变( CIN)Ⅰ级102例、CIN Ⅱ级74例、CIN Ⅲ级36例、宫颈癌16例和尖锐湿疣23例]组HPV阳性率。将细胞学异常组分为4个年龄组：20~岁组85例、31~岁组140例、41~岁组90例、51~岁组110例,比较各年龄组HPV阳性率。结果细胞学正常组HPV阳性率为22.5%(27/120),细胞学异常组为87.3%(371/425)。细胞学异常组及细胞学异常类型6组HPV阳性率分别与细胞学正常组比较,差异均有统计学意义(P<0．01)。细胞学正常组中6种最为常见的HPV基因型按阳性率递减依次为HPV16、68、18、52、58、11；细胞学异常组中6种最常见的HPV基因型依次为HPV 16、52、58、18、33、31。31~岁组、41~岁组HPV阳性率显著高于51~岁组(P<0．01),31~岁组HPV阳性率最高,41岁后随年龄的增长逐渐降低。结论采用导流杂交基因芯片技术一次试验能联合检测出多种HPV亚型感染及型别分布,从而提高由HPV感染引起的妇科肿瘤的防治水平。%Objective To evaluate the clinical value of flow-through hybridization with genechip technology in detection of human papilloma virus ( HPV) of female genital tract, and to explore the most common HPV genotypes and high nosogenic age. Methods The cervical cells of 21 kinds of HPV genotyping assays were performed for 545 cervical cytology specimens using flow-through hybridization with genechip technology. The HPV-positive rates were compared a-mong normal cytology group ( n=120 ) , abnormal cytology group ( n=425 ) and abnormal types of abnormal cytology group 174 cases of chronic cervical inflammation, cervical intraepithelial neoplasia (102 cases of CIN I, 74 cases of CIN II and 36 cases of CIN III) , 16 cases of cervical carcinoma and 23 cases of condyloma acuminatum] . Abnormal cytology group was divided into 4 age subgroups:85 cases of the 20-31 age group, 140 cases of the 31-41 age group, 90 cases of the 41-51 age group and 110 case of older than 51 age group, and HPV infection rates in the four groups were com-pared. Results The HPV-positive rate was 22. 5% (27/120) in normal cytology group and 87. 3% (371/425) in ab-normal cytology group respectively. The differences in HPV-positive rates in abnormal cytology group and 6 subgroups in abnormal types of abnormal cytology group respectively compared with that in normal cytology group were statistically sig-nificant (P<0. 01). Six of the common HPV genotypes in normal cytology group were HPV16, 68, 18, 52, 58 and 11 in turn (by diminishing positive rate);six of the most common HPV genotypes in abnormal cytology group were HPV 16, 52, 58, 18, 33, 31. The HPV-positive rates in the 31-41 age and the 41-51 age groups were significantly higher than that in older than 51 age group (P<0. 01), the 31-41 age group had the highest HPV-positive rate, and the rate gradu-ally decreased with age increasing when the patients were 41 years old. Conclusion The flow-through hybridization with genechip technology may detect a variety of HPV infected subtypes and group types in just one test to improve the level of prevention and treatment of gynecologic cancer induced by HPV infection.

摘要： 目的 研究宫颈癌患者HPV感染情况及基因分布情况,探讨HPV病毒类型与宫颈癌的相关性.方法 收集2008年1月-2011年10月我院确诊的宫颈癌患者103例为调查对象,同期应用基因芯片法检测该患者的23种HPV基因感染情况.结果 103例宫颈癌中有86例患者检测到被HPV感染,感染率为83.5%.结论 HPV基因分型和宫颈癌密切相关,宫颈鳞癌的HPV感染率明显高于宫颈腺癌.HPV16型是宫颈鳞癌和腺癌的主要基因型,宫颈癌中HPV基因分布呈多样性,以高危型为主,HPV16、18型占绝大多数,单纯低危型感染仅占少数.

摘要： 本发明涉及一种用于对借助磁共振指纹法从检查对象采集的磁共振测量数据进行分类的方法、一种分类单元、一种磁共振设备和一种计算机程序产品。用于对借助磁共振指纹法从检查对象采集的磁共振测量数据进行分类的方法包括以下方法步骤：‑采集检查对象的磁共振测量数据，其中磁共振测量数据包括借助磁共振指纹法采集的多个磁共振信号走向，‑从磁共振测量数据推导出至少一个纹理参数，‑利用至少一个纹理参数将磁共振测量数据分类为至少一个组织类别，和‑提供分类后的磁共振测量数据。

摘要： 本发明公开了一种通过聚合酶链式反应（PCR）检测样品中目标病原体DNA并对其进行基因分型的分子指纹法。该方法包含进行PCR扩增和随后的高分辨率熔解（HRM）分析。所用的PCR反应混合物包含两对或更多对扩增引物，其被设计来产生彼此具有不同熔解温度的扩增子，以便在HRM分析中通过观察每个扩增子的特异性的熔解温度来区分每个扩增子。该方法还包含监测在HRM分析过程中由于嵌入分子或化合物的释放，温度诱导的双链扩增子变性为两个单链DNA而产生的信号发射变化。通过读取器分析信号变化以确定样品中同一病原体的不同株、属于不同属和遗传变异的不同病原体的区分和基因分型，分析结果通过连接到读取器的图形界面获得。

摘要： 本发明涉及一种用滞留层析法捕获蛋白质组进行分析的蛋白指纹法。本方法用质谱法进行鉴别检测蛋白质组。分析所得数据的形式是可用计算机读取的条码格式(蛋白指纹)。本发明的方法可用于检测血液中正常人细胞及肾癌患者细胞的蛋白指纹。本方法准确、方便且快捷。

摘要： 本发明涉及一种用滞留层析法捕获蛋白质组进行分析的蛋白指纹法。本方法用质谱法进行鉴别检测蛋白质组。分析所得数据的形式是可用计算机读取的条码格式(蛋白指纹)。本发明的方法可用于检测血液中正常人细胞、不稳定型心绞痛细胞及急性心肌梗死患者细胞的蛋白指纹。本方法准确、方便且快捷。

摘要： 本发明涉及一种用滞留层析法捕获蛋白质组进行分析的蛋白指纹法。本方法用质谱法进行鉴别检测蛋白质组。分析所得数据的形式是可用计算机读取的条码格式(蛋白指纹)。本发明的方法可用于检测血液中正常人细胞及胰腺癌患者细胞的蛋白指纹。本方法准确、方便且快捷。

摘要： 本发明涉及一种用滞留层析法捕获蛋白质组进行分析的蛋白指纹法。本方法用质谱法进行鉴别检测蛋白质组。分析所得数据的形式是可用计算机读取的条码格式(蛋白指纹)。本发明的方法可用于检测血液中正常人细胞、结直肠癌患者细胞及结直肠息肉病患者细胞的蛋白指纹。本方法准确、方便且快捷。

摘要： 本发明涉及一种用滞留层析法捕获蛋白质组进行分析的蛋白指纹法。本方法用质谱法进行鉴别检测蛋白质组。分析所得数据的形式是可用计算机读取的条码格式(蛋白指纹)。本发明的方法可用于检测血液中正常人细胞及胃癌病患者细胞的蛋白指纹。本方法准确、方便且快捷。

摘要： 本发明涉及一种用滞留层析法捕获蛋白质组进行分析的蛋白指纹法。本方法用质谱法进行鉴别检测蛋白质组。分析所得数据的形式是可用计算机读取的条码格式(蛋白指纹)。本发明的方法可用于检测尿液中正常人与病人(如肾病综合症及红斑狼疮性肾炎)的尿蛋白指纹。本方法准确、方便且快捷。

摘要： 以各种方式组合内容指纹和水印以便用于内容鉴别应用场合。使用指纹来一般地鉴别内容，同时水印提供内容内的各部分的更详细的定位，反之亦然。还使用指纹技术进行信号同步和其它预处理步骤以便帮助进行数字水印解码。各种指纹/水印技术从内容样本鉴别内容的通道特性。

摘要： 本发明提供了一种应用于管道电场指纹法腐蚀检测技术的螺柱焊工装，属于焊接技术领域，有效解决了管道监测探针焊接定位不准影响监测性能等问题。该技术方案包括两个相对应设置同时套设于管道外侧的套环，每个套环外壁固定连接有与管道配合的若干夹持件，两个套环之间设置有滑动块，滑动块上设置螺柱焊枪工位，滑动块连接有平移机构，平移机构连接有转动机构。本发明的有益效果为：提供了一种可以保证管道螺柱焊接定位精准，焊接效果一致、美观的螺柱焊工装。