珍珠菜

摘要： 古人认为:物以稀为贵,食以野为奇。野菜是自然界长期选择的产物,抗逆性强,病虫害少,栽培种植时不施化肥、不打农药,具备洁净、绿色、保健之功能。南京的野菜中,大部分是我国特有品种,很多还是名贵的中药材。如香椿有清热解毒、健胃理气、杀虫之效,民间有“常食香椿不生杂病”之说;冬寒菜有利尿润肠之功;独行菜有较强的防癌活性;珍珠菜可治皮肤热毒、目赤肿痛;枸杞可调节免疫,延缓衰老。

摘要： 春暖花开,又是三月女人天。珠宝饰品总是与女人紧紧联系在一起,女人们因珠宝饰品更动人,而珠宝饰品也因女人更璀璨。珠宝首饰不仅可以记录女人们每一个人生精彩阶段的心路历程,也为女人们带来自信与魅力!让我们一起关心身边的她或她,让她们在女人节这天再次绽放光芒。

摘要： 对珍珠莱Lysimachia clethroides的化学成分进行研究.应用硅胶、Sephadex LH-20和制备型HPLC等柱色谱技术进行分离纯化,并运用现代波谱技术(ESI-MS,1H NMR,13C NMR)进行结构鉴定.从珍珠菜中分离得到12个化合物,分别鉴定为山柰酚(1)、槲皮素(2)、cinchonain Ib(3)、芹菜索-6-C-β-D-吡喃木糖基-8-C-α-L-吡喃阿拉伯糖苷(4)、芹菜素-6,8-二-C-α-L-吡喃阿拉伯糖苷(5)、芹菜素-6-C-α-L-吡喃阿拉伯糖基-8-C-β-D-吡喃木糖苷(6)、芹菜素-6,8-二-C-β-D-吡喃木糖苷(7)、芹菜素-6-C-β-L-吡喃阿拉伯糖基-8-C-β-D-吡喃葡萄糖苷(8)、芹菜素-6-C-α-L-吡喃阿拉伯糖基-8-C-β-D-吡喃葡萄糖苷(9)、芹菜素-6-C-β-D-吡喃葡萄糖基-8-C-α-L-吡喃阿拉伯糖苷(10)、blumenolA (11)、(3S,5R,6R,7E,9S)-大柱香波龙-7-烯-3,5,6,9-四醇3-O-β.D-吡喃葡萄糖苷(12).化合物3～8和11 ～12为首次从该属植物中分离得到.体外活性测试结果显示化合物1～12均没有明显的细胞毒活性(IC50＞ 10 μM).

摘要： 以珍珠菜种子为材料进行消毒及无菌苗培养,再以消毒后萌发的无菌苗的叶片、茎段和带叶腋茎为外植体,开展丛生芽的诱导、增殖培养、生根培养和移栽驯化技术研究。结果发现,珍珠菜种子最佳消毒方式为5%次氯酸钠消毒10 min,最适丛生芽诱导部位为叶片,丛生芽诱导最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.05 mg/L；其次为MS+6-BA 2.0 mg/L+NAA 0.05 mg/L。最适珍珠菜增殖的培养基为MS+6-BA 1.0 mg/L,其次为MS+6-BA 1.0 mg/L+KT 2.0 mg/L。最适生根培养基为1/2MS+NAA 0.5 mg/L,其次为1/2MS+NAA 1.0 mg/L。最适珍珠菜移栽驯化的方法为开瓶驯化5 d后种植于蛭石∶珍珠岩∶泥炭(1∶1∶1)基质中。本研究结果为利用组织培养技术实现珍珠菜快速繁殖和人工扩大栽培奠定了良好基础。

摘要： [目的]对安庆地区省沽油物候期和形态特征进行观察探究,为省沽油在沿江丘陵地区推广种植、开发利用提供理论和技术基础.[方法]对移栽4年生40株省沽油的物侯期、形态特征进行系统调查.[结果]安庆地区省沽油2月下旬～3月上旬萌动；3月上旬～4月中旬展叶、现蕾；4月初～5月初进入开花期；4月底～6月上旬结果；8月上旬～9月下旬果实成熟；8月中旬～10月初蒴果脱落；10月下旬～11月下旬叶片变色、脱落；随后冬季枝干进入休眠期,到第2年2月上旬.以4年树龄省沽油为例,平均株高148 cm,最高株高1.7m,平均地径2.0 cm,每株平均主干枝6根,每根主干枝平均着生8根果枝,当年生长量为40～ 80 cm.圆锥花序顶生直立,形似珍珠,蒴果膀胱状,种子椭圆形而扁.[结论]省沽油在沿江丘陵坡地可栽培.%[ Objective ] By researching phenological phase and morphological characteristics of Staphylea bumalda DC in Anqing, the study provides theoretical and technical basis for popularizing planting and exploiting Staphylea bumalda DC in hill area along Yangtze River. [ Method ] A systematic investigation was made on phenological phase and morphological characteristics of forty strains of four-year-old Staphylea bumalda DC. [ Result] The sprouting stage of Staphylea bumalda DC in Anqing was from late February to early March , the expending leaf and bud stage was from early March to mid-April, the blooming stage was from early April to early May, the fruiting stage was from late April to early June, the fruit ripening stage was from early August to late September, the capsule shedding stage was from mid-August to early October, yellow and fall off of leaf was from late October to late November, and the dormancy stage was from winter to the following early February. Take example for four-year-old Staphylea bumalda DC, its average height was 148 cm with the highest of 1. 7 m, the average ground diameter was 2.0 m , the average main branches of per plant were six roots, the average fruit branches of per main branch were eight roots, the current annual increment was 40 cm to 80 cm. And its pearly panicle was upright on the top, capsules shape was bladdery, seed's shape is elliptic. [ Conclusion ] Staphylea bumalda DC can be planted in hilly upland along Yangtze River.

摘要： Objective To investigate the effect of total flavonoids from Lysimachia clethroides Duby(ZE4) on human leukemia HL-60 cell xenograft in nude mice.Methods After HL-60 cells were inoculated subcutaneously in nude mice to establish xenograft models of leukemia in 30 mice,the mice were equally randomized into 5 groups.The mice in groups of A,B and B were intraperitoneally injected with ZE4 100,200 and 400 mg/kg,respectively,those in group D with CTX 50 rng/kg,and those in group E with normal saline as blank controls.The injection was performed every other day for 21 days.The inhibitory effect of ZFA was observed and immunohistochemistry was used to detect the positive expressions of Ki-67 and CD34 in the xenografts.Results Compared with group E,the growth of xenografts was significant1y inhibited in groups of B and C with the inhibition rates of 51.32％ and 46.06％,respectively(P＜0.05).Immunohistochemistry showed that the expressions of Ki-67 and CD34 in the xenografts in groups of B and C were obviously less than those in group E.Conclusion ZE4 has a significant inhibitory effect on leukemia,which might be related to the suppression of angiogenesis and proliferation of tumor cells.%目的 研究珍珠菜总黄酮(ZE4)对白血病HL-60细胞裸鼠皮下移植瘤的抑制作用.方法 成功建立人白血病HL-60细胞皮下移植瘤模型(移植瘤体积约100 mm3)后的30只裸鼠均分为五组:A、B、C组分别腹腔注射ZE4 100、200、400 mg/kg;D组注射环磷酰胺(CTX) 50 mg/kg;E组以生理盐水对照;每2天注射一次.21d后测量移植瘤大小,采用免疫组化法检测移植瘤组织中细胞增殖抗原Ki-67和血管内皮细胞膜抗原CD34的表达水平.结果 与E组比较,B和C组移植瘤生长明显抑制,抑瘤率分别可达51.32％和46.06％;免疫组化结果显示,B、C组移植瘤中Ki-67和CD34的表达明显少于E组.结论 ZE4对白血病HL-60细胞裸鼠移植瘤具有较明显的抑制作用,可能与其抑制肿瘤细胞增殖和肿瘤新生血管形成相关.

摘要： Objective To study the application of optimization analysis of Vector Evaluated Genetic Algorithm(VEGA) on extraction technology of active ingredients for Anti-tumor in Lysimachia clethroides Duby. Methods To establish sub-target model of total flavonoids and total Saponin on extraction technology of active ingredients for Anti-tumor in Lysimachia clethroides Duby experimental data in orthogonal design. Applying VEGA explores the optimal extracting conditions and compare results with Single-objective genetic algorithm. Using SGALAB beta5OO8 of the Matlab2009a plug-in tool-box, which was written by Chen Yi in the U-niversity of Electronic Science and Technology, achieves the genetic algorithm optimization; SPSS13. 0 software was used for statistical analysis. Results The optimal conditions for the ethanol concentration 80% , 8 times of ethanol dosage, extraction times is 1, extracting time according to the actual situation. Under these conditions, the results of the total flavonoids is 10. 36% , the total Saponin is 25. 79% . Conclusion VEGA provides a reasonable pareto ensuring the optimal multi-objective, which provides a reasonable method of choice for the active ingredients for Anti-tumor in Lysimachia clethroides Duby. This method can be extended to the selection of other drugs.%目的 研究向量评估遗传算法(VEGA)在珍珠菜抗肿瘤有效成分提取工艺条件中的应用,并对其效果进行评价.方法 对珍珠菜抗肿瘤有效成分提取工艺正交设计试验数据建立以总黄酮含量和总皂苷含量的子目标模型,采用VEGA对其进行多目标优化,搜索最优提取条件.利用课题组成员电子科技大学软件工程师陈益提供的Matlab2009a外挂SGALAB工具箱beta5008完成遗传算法的寻优；利用SPSS13.0软件进行统计分析.结果 得到的最优工艺条件为80%乙醇,8倍用量,提取1次,提取时间根据实际情况选择的条件下的搜索总黄酮含量为10.36％、总皂苷含量25.79％.结论 VEGA收索的Pareto非劣解在保证多目标都最优的前提下是比较理想的,为珍珠菜抗肿瘤有效成分的最优条件选择提供了合理的方法,可推广到其他药物的最优条件选择.

摘要： OBJECTIVE To study the inhibition of ZE4, the antitumor effective part from Lysimachia clethroides Duby, on Bel-7402 cell xenografted hepatoma and its possible mechanism. METHODS The sc transplanted tumor model of Bel-7402 cells in nude mice was established and then divided randomly into five groups, including model group and ZE4 100, 200 and 400 mg·kg-1 and 5-fluorouracil (5-FU) 20 mg·kg-1 groups. The mice were ig given ZE4 once daily or ip given 5-FU every two days, for 21 d. The tumor volume was measured every three days after transplantation of Bel-7402 cells before the growth curve was made. All the mice were sacrificed after the last administration. The masse of the dissected tumor tissue were mearsured and the inhibitory rate was calculated. The expression of apoptotic protein Bax and Bcl-2 in transplanted tumor tissue was detected using Western blotting. CD34 expression in transplanted tumor tissue was detected with im-munohistochemistry and microvessel density (MVD) was counted. RESULTS ZE4 could inhibit the growth of transplanted tumor in nude mice after administration for 21 d. The transplanted tumor mass in ZE4 200 mg·kg-1 group was(1.5 ±0.5)g, which significantly decreased (P＜0. 01) compared with that of model group (3.2 ±1.0) g. The inhibitory rate was 52. 1%. The transplanted tumor volume in ZE4 200 mg·kg-1 group was (0.83 ±0.27) cm3 after administration for 14 d, which was smaller than in model group (1. 74 ± 0. 67 ) cm3 ( P ＜0. 05 ) . After administration for 21 d, the transplanted tumor volume in ZE4 200 mg·kg-1 group( 1.40 ±0.42) cm3 was also significantly smaller than that in model group (2. 90 ± 0. 89 ) cm3 ( P ＜0. 05). Compared with model group (0. 253 ±0. 008) , the ratio of Bax/Bcl-2 (1.157 ±0. 058) in ZE4 200 mg·kg-1 group significantly increased (P＜0. 01) , but there was no significant change in ZE4 100 and 400 mg·kg-1 groups. Immunohistochemistry analysis showed ZE4 100, 200 and 400 mg·kg-1 could effectively reduce an-giogenesis. ZE4 200 mg·kg-1 reduced MVD to 13.5 ±1.0 from model group(42. 3 ±2. 5) mm-2 (P＜0.01). CONCLUSION ZE4 has significant inhibition on Bel-7402 cell transplanted tumor in nude mice, which may be related to its promotion of apoptosis and reduction of angiogenesis.%目的 研究珍珠菜抗肿瘤有效部位ZF4对肝癌Bel-7402细胞裸鼠移植瘤的抑制作用及可能机制.方法 制备肝癌Bel-7402细胞裸鼠移植瘤模型,裸鼠分别ig给予ZE4 100,200和400 mg· kg-1每天1次,或ip给予5-FU 20 mg·kg-1,隔日1次,共21 d.移植肿瘤细胞后每3d测量移植瘤体积并绘制生长曲线,末次给药后处死小鼠,剥离移植瘤组织称瘤质量并计算抑瘤率.应用Western印迹法检测移植瘤组织匀浆中凋亡相关蛋白Bax和Bcl-2蛋白表达,免疫组织化学法检测移植瘤组织中CD34表达,计数微血管密度(MVD).结果 ZF4可抑制裸鼠移植瘤的生长,给药21 d后,ZE4 200 mg· kg -1组瘤质量为(1.5±0.5)g,较模型组(3.2±1.0)g明显减轻(P＜0.01),抑瘤率达52.1％；移植瘤体积在给药14 d后为(0.83±0.27) cm3,与模型组(1.74±0.67) cm3相比,明显减小(P＜0.05),给药21 d后为(1.40±0.42) cm3,比模型组(2.90±0.89) cm3相比,明显减小(P＜0.05).ZE4 200 mg·kg-1组Bax/Bcl-2比值(1.157±0.058)较模型组(0.253±0.008)显著升高(P＜0.01),ZE4 100和400 mg· kg -1组无明显变化.ZE4 100,200和400 mg·kg -1组血管生成均明显减少,其中ZE4 200 mg·kg-1组MVD( 13.5±1.0)较模型组(42.3±2.5)mm-2明显降低(P＜0.01).结论 ZF4对肝癌Bel-7402细胞裸鼠移植瘤具有明显抑制作用,可能与促进细胞凋亡和抑制血管生成有关.

摘要： 目的优选珍珠菜中芦丁、异槲皮苷、山柰酚3-O-β-D-半乳糖苷及紫云英苷的最佳提取工艺.方法通过正交试验设计考察甲醇浓度、提取时间、提取温度、料液比对珍珠菜中4种黄酮含量的影响.结果最佳提取工艺条件为70%甲醇,提取时间45 min,提取温度45°C,料液比（g/mL）1：100.结论该工艺稳定、有效,简单易行.

摘要： 本发明提供一种珍珠白玉丸菜品包及其制备方法，属于冷冻食品技术领域。珍珠白玉丸菜品包的制备方法，包括如下步骤：(1)、浸泡木耳和香菇并去掉水分，将木耳、香菇和肉类通过绞肉机绞碎，加入配料腌制得到肉馅。(2)、将肉馅制成肉丸，并在肉丸表面覆盖泡制过的糯米得到丸子。(3)、将丸子汽蒸、冷却、冷冻并分袋包装。此制备方法制得的珍珠白玉丸菜品包方便食用、营养价值高，水分较少且口感好。

摘要： 本发明涉及中药材应用领域，具体而言，涉及虎刺和/或珍珠菜在制备治疗再生障碍性贫血药物中的应用。本发明提供了虎刺总黄酮和/或珍珠菜总黄酮在制备治疗再生障碍性贫血药物中的应用。本发明首次发现了虎刺和/或珍珠菜对再生障碍性贫血具有一定的疗效，特别是虎刺总黄酮和/或珍珠菜总黄酮对再生障碍性贫血具有良好的疗效。

摘要： 本发明公开了一种珍珠菜保健酱菜的加工工艺，其特征在于：采用原料处理→杀青、保脆→冷冻干燥→盐腌→过滤、真空浓缩→酱汁调制→真空酱渍→包装、巴氏杀菌→检验、成品为加工工艺流程。有益效果：本发明以绿色自然、营养丰富的珍珠菜为原料，通过杀青、保脆、冷冻干燥、真空浓缩、真空酱渍、巴氏杀菌等方法，使酱菜脆嫩爽口、营养丰富，还具有健脾开胃、清热利湿等功效，是一种绿色健康、开胃滋补的保健酱菜。老少皆宜，食用方便。

摘要： 本发明公开了一种珍珠菜浓缩汁的加工方法，所述的珍珠菜浓缩汁以新鲜成熟的珍珠菜为原料，进行杀青、微冻处理、微波干燥、超声波提取、回流浸提、混合、低温浓缩、杀菌等加工工序，保留了原料所含的营养成分，使加工出的成品营养丰富，口感细腻，具有清热利湿等保健功效，提高了珍珠菜的营养价值和经济价值，同时也为珍珠菜的开发利用提供一条新的途径。

摘要： 本发明属于食品保健技术领域，公开了一种珍珠菜保健膏的加工方法，所用原料主要有珍珠菜、抗坏血酸钠溶液、纤维素酶、果胶酶、诃子果、苹果酸溶液、杜仲叶、硒水溶液，其中抗坏血酸钠溶液浓度为5％，苹果酸溶液的浓度为20％，硒水溶液的浓度为7％。本发明加工的保健膏以绿色健康、营养丰富、来源广泛的珍珠菜和诃子果为主要原料，不仅降低了生产成本，而且丰富了保健膏的新品种及保健膏的营养成分，携带方便、色泽稳定、口感纯正、提高了经济效益，具有镇痛健胃、补中益气、保肝利胆、抗氧化等功效。该方法能够提高保健膏的营养价值和经济价值，丰富了珍珠菜产品的种类。

摘要： 本发明公开了一种珍珠菜有效成分的制备方法，步骤如下：将珍珠菜的干燥全草用体积分数为55‑65%的乙醇在微波萃取仪中提取3‑5次，每次40‑50min，微波温度为50‑60°C，微波功率为450‑550W，提取结束后过滤，减压浓缩得到浸膏；浸膏用适量水溶解后经大孔吸附树脂柱分离，依次用水、25%、45%、65%、75%、95%的乙醇梯度洗脱，收集65%乙醇洗脱部位，再经硅胶柱色谱分离，依次用体积比90:1、72:1、36:1、13:1、5:1的氯仿‑甲醇洗脱，收集13:1洗脱部分，浓缩即得。该方法可快速得到珍珠菜中的有效成分，具有显著的抗菌消炎活性，可大规模制备。

摘要： 本发明公开了一种特色珍珠菜保健食品的制作方法，属于食品加工领域。其特征在于：采用的加工工艺流程为选料→整理清洗→切段烫漂→脱水干燥→浸渍调味→包装。有益效果：本发明产品香辣爽口，口感脆嫩、且带有弹性，具有珍珠菜特有的清甜风味，营养丰富；本产品富含营养成分，有助于增强机体免疫力，具有清热利湿、活血散瘀、解毒消痈等功效，是一种绿色健康、营养美味的风味食品。

摘要： 本发明公开了一种珍珠菜保健脯的加工方法，属于食品加工领域。其特征在于：采用选料→去杂清洗→切段烫漂→糖制→冷却→包装的加工工艺流程。有益效果：本发明产品清脆爽口、口感细腻、组织脆嫩，具有珍珠菜特有的清甜风味，营养丰富；本产品富含营养成分，有助于增强机体免疫力，具有清热利湿、活血散瘀、解毒消痈等功效，是一种营养健康的纯天然绿色食品。

摘要： 本发明公开了一种珍珠菜鲜榨真空干燥粉末饮片的制备工艺，其特征在于，包括以下步骤：1)预处理；2)压榨取汁；3)混合过滤；4)浓缩；5)干燥制粒；6)包装；本发明公开的制备工艺，不仅可以减少饮片体积，最大限度保留珍珠菜药材中的极性和非极性有效成分，还可以避免药材在现有饮片加工和储运过程中引起的有效成分的丢失和药材品质的下降，减少药材染菌风险。本发明对确保珍珠菜的质量稳定和临床安全有效具有重要的意义。

摘要： 本实用新型属于珍珠菜加工设备技术领域，公开了一种用于珍珠菜保健膏制备的搅拌装置，搅拌罐顶部设置有罐盖，罐盖上端侧面开设有搅拌罐真空口，搅拌罐真空口与真空泵连通，罐盖中间通过螺栓安装有电机，电机输出轴下端与搅拌罐内的搅拌棒联接，搅拌棒外周焊接有多个螺旋状排列的搅拌叶片；搅拌罐下端连通有乳化罐，乳化罐下端侧面开设有物料出口，物料出口内侧嵌装有均质头，乳化罐侧面上端开设有与充气泵连通的充气口。本实用新型将物料通过搅拌罐内的搅拌杆进行搅拌，物料在搅拌过程中产生的气泡被及时抽走，使所生产的制品在搅拌过程中不再混入气泡，通过螺旋状排列的搅拌叶轮可以将物料不断的向下输送挤压，避免物料粘附在内壁上。