柽柳核基因组微卫星反应体系的建立和优化

摘要

Tamarix chinensis Lour. is a salt-tolerance shrub or small tree,scattered in the riverbed or forming large natural populations in coastal tidal areas. For population genetics study of this species,the orthogonal design was used to determine the PCR amplification system of genomic SSR derived from Illumina sequencing in T. chinensis. The 10卜L reaction system of gSSR is as follows:0.8 mmol/L Mg2+,0.25 mmol/L dNTPs,0.5 U Taq DNA polymerase,0.5卜mol/L primers,and 20 ng DNA.%柽柳(Tamarix chinensis Lour.)是耐盐碱的灌木或小乔木,多散生于河漫滩或在沿海滩涂形成大规模群体.对由Illumina测序开发的柽柳微卫星标记反应体系进行了正交试验设计,确定10卜L SSR-PCR反应体系的各反应物浓度为Mg2+0.8 mmol/L、dNTPs 0.25 mmol/L、Taq DNA聚合酶0.5 U、引物0.5卜mol/L、模板DNA 20 ng.