异地板蓝根基因组DNA指纹图谱建立及RAPD-PCR反应体系优化

摘要

Objective:RAPD method is used to study Radix Isatidis genomic DNA genetic difference and the true and false of the medicinal materials in different regions.Methods:Use RAPD technology for Radix Isatidis genomic DNA genetic diversity analyses at the molecular level from different regions, and optimize the Radix Isatidis RAPD-PCR reaction system.Results:Fingerprints showed in different regions Radix Isatidis resource had rich genetic diversity, and could distinguish between true and false, and the pros and cons of herbs.Conclusion:RAPD technique for the identification of the authenticity and the pros and cons of Chinese herbal medicines Radix Isatidis provides a practical significance for clinical safety and provide a theoretical basis for rational drug use.And Radix Isatidis best RAPD-PCR reaction system has been established.%目的：采用RAPD法研究不同地区板蓝根基因组DNA遗传差异性，药材的真假。方法：利用RAPD（ random amplified polymorphic DNA）技术，在分子水平上对不同地区板蓝根基因组DNA进行了遗传差异性分析，同时优化板蓝根RAPD-PCR反应体系。结果：指纹图谱显示出不同地区板蓝根资源丰富的遗传多样性，可以辨别出药材的真假和优劣。结论：RAPD技术为鉴别中药材板蓝根真伪和优劣提供了现实意义，为临床安全和合理用药提供理论依据，并建立适合板蓝根RAPD-PCR的最佳反应体系。