Determination of Water-Soluble Polyphenols Compounds in Commercial Herbal Teas from Lamiaceae:Peppermint,Melissa,and Sage

摘要

Chromatographic techniques (HPLC and HPTLC) were used for qualitative and quantitative determination of eriocitrin,luteolin 7-O-rutinoside,luteolin 7-O-beta-glucuronide,lithospermic acid,rosmarinic acid,and methyl rosmarinate together with other known compounds in commercial herbal teas from the Lamiaceae family:peppermint leaf (Menthae piperitae folium),melissa leaf (Melissae folium),and sage leaf (Salviae officinalis folium).Contents of analyzed compounds in infusions,the most popular forms,were established using a C18 column with acetonitrile-water-formic acid as a mobile phase.The HPLC method was validated for linearity,precision,and accuracy.Luteolin 7-O-beta-glucuronide and lithospermic acid were identified as new Mentha x piperita compounds.The investigated herbal teas delivered polyphenols in high amounts,up to 182.2 mg for the infusion of one peppermint tea bag.