HPLC法同时测定高冠平胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量

摘要

目的:建立高效液相色谱法同时测定高冠平胶囊中三七、红参所含皂苷类成分含量的方法.方法:采用高效液相色谱法,色谱柱为HibarC18(250mm×4.6mm,5μm);流动相A为乙腈,B为水;流速为1.0mL·min-1;检测波长为203nm;洗脱程序:0～35min,A相为19%,B相为81%;35～85min,A相为19%→35%,B相为81%→65%;85～100min,A相为35%→19%,B相为65%→81%.结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.4104～3.2832μg、2.0884～16.7072μg、1.222～9.776μg、2.0072～16.0576μg范围内呈良好的线性关系,其回收率分别为98.92%、98.97%、99.01%、99.25%,RSD分别为1.08%、1.06%、0.85%、1.15%.结论:本方法简便、准确、重现性好,可用于高冠平胶囊的质量控制.%Objective: To establish an HPLC method for determination of Saponins of Notoginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra in Gaoguanping capsules. Methods:The HPLC was performed on Hibar C18( 250mm ×4. 6mm ), acetonitrile as mobile phase A,water as mobile phase B. The flow rate was 1. 0 mL · min-1. The detection wavelength was 203 nm. Gradient elution: 0-35 min, A: 19% , B: 81% ; 35 ~ 85 min,A:19% →35% ,B: 81% →65% ;85 ~100min, A:35%→19% , B:65→81%. Results: The linear range of notoginsenoside R, , ginsenoside Rg, ginsenoside Re and ginsenoside Rb, was 0.4104 ~ 3.2832μg, 2.0884 ~ 16. 7072μg, 1. 222 ~ 9. 776μg, 2. 0072 ~ 16. 0576μg with good con-elation. The average recovery was 98. 92% , 98. 97% , 99. 01% , 99. 25% , RSD wasl. 08% .,, 1. 06% , 0. 85% , 1. 15%. Conclusion: The method is simple, accurate and reproduciable. It can be used for quality control of notoginsenoside R, , ginsenoside Rg, , ginsenoside Re and ginsenoside Rb, in Gaoguanping capsules.