HPLC法测定不同产地西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量

摘要

Objective: To detect ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 of three active principles in American ginseng from different regions by HPLC. Methods: A C18 column was used by gradient elution of acetonitrile -0.1％ H3PO4 solution as the mobile phase, the flow rate was 1.1 ml/min and the detective wavelength was set at 203 nm. the column temperature was seL at 30℃. Results: All of the three compounds showed good linearities and recoveries were in the range of 97.2％-99.0％ with RSD of 1.61％-2.03％. Conclusion: The method is accurate and suitable for the determination of ginsenoside Rg1, ginsenoside Re, ginsenoside Rh1.%目的:对不同产地西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1三种主要药效成分进行高效液相色谱的含量测定.方法:采用C18柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速为1.1 ml/min,检测波长为203 nm,柱温为30℃.结果:人参皂苷Rg1、Re、Rb1、在测定范围内有良好的线性关系,其平均回收率为97.2%～99.0%,RSD为1.61%～2.03%.结论:该方法准确可靠,重现性好,应用性强.