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DESIGN OF BIAPTAZYME WITHOUT SELF-CLEAVAGE ACTIVITY OF RIBOZYME AND ITS APPLICATION TO BIOSENSOR OR BIOCHIP DEVELOPMENT
DESIGN OF BIAPTAZYME WITHOUT SELF-CLEAVAGE ACTIVITY OF RIBOZYME AND ITS APPLICATION TO BIOSENSOR OR BIOCHIP DEVELOPMENT
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机译:不具有核酶自我裂解活性的双歧酶的设计及其在生物传感器或生物芯片开发中的应用
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摘要
The present invention has been developed an application of the sensor of this application as bio-materials and bio-chip and the microfluidic chip belongs to the sensing system (sensing), detection, diagnosis, and gene therapy in the field of biological material. ; In developing that Ephesians Tamer and ribozymes combination of Ephesians hitter, in the Ephesians hitters developed in previous studies Lim showed an absence, self-decomposition reaction of the target substance, the decomposition only party in the ribozyme If you have a target substance in the present invention, system response to the animations (stem) by creating a design that Fusion (fusion) with the Tamer in Ephesians and I systems III, more sensitive and more active good-by-design approach was developed Ephesians any batsman. Use them in developing new Eph batter Im chip system for real-time as proteins, cells, and biological sensing the material, etc., and determine the detected substance to double over the mass analyzer again through the fluorescence within the microfluidic chip system multi-channel will be. ; Purpose of the invention is to develop a biosensor for detecting a biological material and diagnosis without signal noise, first, This can be used as a sensor that can detect a specific substance, the fluorescence when applied to a bio-chip to the microfluidic chip, and by using the mass spectrometer can be two-dimensional analysis can be used in real-time diagnosis and analysis or the like. ; In addition to the study of gene therapy that can be developed through a design to cut a specific gene by an external matter it can be effectively applied. ; Ribozymes, Tamer Ephesians, Ephesians hitter Im bi-hitter Ephesians Lim, biochips
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