DEVELOPMENT OF A GLUTAMATE BIOSENSOR BASED ON GLUTAMATE OXIDASE USING SMART-BIOCHIPS

摘要

A highly sensitive L-glutamate biosensor based on glutamate oxidase (GLOx) has been fabricated on N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) activated thioglycolic acid (TGA) self assembled monolayer (SAM) on smart-biochips, where K_3Fe(CN)_6 and TGA-SAM are used as a mediator and an enzyme immobilization matrix, respectively. The glutamate biosensor displayed a fast response, a low detection limit (DL, 0.072 μM), a wide linear dynamic range (LDR, 0.1 μM to 10.0 mM), good linearity (R=0.9843), and higher sensitivity (17.89 μAmM~(-1)cm~(-2)), small sample volume (70.0 μl), as well as good stability and reproducibility. The Au/TGA system exemplified a simple and efficient approach to the assimilation of GLOx and electrodes, which can provide analytical access to a large group of enzymes for wide range of bio-electrochemical applications in health care fields.