APPLICATION OF ANTHER CULTURE AND MOLECULAR MARKERS TO A PEPPER BREEDING PROGRAM FOR DISEASES RESISTANCE.

摘要

Pepper (Capsicum annuum L.) is one of the most important vegetable crops in Spain Virus diseases are among the main constraints of pepper production. In addition to viral diseases, Phytophthora capsici Leon, is the most serious fungal disease in temperate areas. Therefore, breeding pepper varieties with joint resistance to diverse pathogens is an objective of any selection programme. At the present time several disease resistance sources in pepper are known. The C. annuum variety 'Serrano Criollo deMorelos-334 ('SCM-334') has high levels of resistance to P. capsici and Potato Virus Y (PVY, gene Pvr4), as well as to Tomato Mosaic Virus (ToMV, gene L1). Another well-known source of resistance to P. capsici is the C. annuum line 'Pf-201234'. For years, our group has developed six lines with joint resistance to P. capsici, ToMV and PVY (pathotype 1-2), using as recurrent parent lines of the types 'Yolo Wonder' (YW) and 'Morron'. Nevertheless, the breeding processes are long and expensive, reasons whythe use of anther culture and molecular markers linked to resistance genes would facilitate them. Therefore, the objective of this work was to obtain from the six previously mentioned Sines, several dihaploides lines (DH) to be used as hybrid parents resistant to the three above mentioned pathogens. In addition, the resistance to PVY-1-2 was evaluated by means of molecular markers related to the Pvr4 locus.