Inter- and intra-population genetic variations in Jatropha curcaspopulations revealed by inter-simple sequence repeat molecularmarkers

摘要

Inter simple sequence reaction (ISSR) was employed to assess diversity in six Jatropha populations that were collectedin Malaysia, Indonesia, the Philippines and India. A total of 144 accessions (24 accessions per population)were selected and analysed with 10 ISSR primer combinations to generate a total of 143 polymorphic fragments.The number of bands generated per primer varied from 4 to 27. The percentages of polymorphic bands for theIndonesia1, Indonesia2, Malaysia1, Malaysia2, Philippines and India Jatropha populations were 54.6, 59.4, 46.2,53.2, 60.8 and 56.4%, respectively, with an average of 55.1%. The Nei’s total genetic diversity (HT), the intra-populationsgenetic diversity (HS) and the coefficient of genetic differentiation (GST) were 0.1136, 0.0989 and 0.1295,respectively. The coefficient of genetic differentiation (GST) was 0.1295, which indicated a frequency of approximately13% in genetic variations observed in the inter-Jatropha populations, while an 87% variation correspondedto the intra-Jatropha populations. Analysis of molecular variance (AMOVA) analysis indicated highly significant (p ≤0.001) genetic differences among the six Jatropha populations. An UPGMA dendrogram was constructed, and theJatropha populations were grouped into four major clusters at a coefficient level of 0.28. The genetic similaritiesbetween the populations ranged from 0.31 to 0.25. The principal component analysis showed a relatively similargrouping of the populations. To obtain high heterotic responses, hybridisation should be made between the twodistant populations of Malaysia 1 and Indonesia1.