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Extraction of Protein from Yellowfin Tuna (Thunnus albacares) Waste by Enzymatic Hydrolysis and its Characterization

机译:酶解法从黄鳍金枪鱼废物中提取蛋白质及其表征

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The focus of this investigation was to utilize the protein present in tuna waste by enzyme hydrolysis and characterization of protein hydrolysate (TPH). Yellowfin tuna waste was used to prepare the hydrolysate using 0.5% (w/w) bromelain enzyme. It wascharacterized for nutritional, functional and antioxidant properties. The protein content in the derived protein hydrolysate was 95.3 ± 3.1%. The foaming capacity as well as foam stability was maximum at pH 6.0 and the property decreased when pH deviated from neutral. Emulsifying properties viz., emulsifying activity index as well as emulsion stability index was least at 4.0 pH. DPPH radical scavenging activity of TPH revealed an IC50 value of 4.06 mg ml"1.
机译:这项研究的重点是通过酶水解和表征蛋白水解物(TPH)来利用金枪鱼废物中存在的蛋白。使用黄鳍金枪鱼废物,使用0.5%(w / w)的菠萝蛋白酶来制备水解产物。它具有营养,功能和抗氧化特性。衍生的蛋白质水解物中的蛋白质含量为95.3±3.1%。在pH 6.0时,发泡能力和泡沫稳定性达到最大,并且当pH偏离中性时,性能下降。乳化性质即乳化活性指数以及乳化稳定性指数至少在4.0pH下。 TPH的DPPH自由基清除活性显示IC50值为4.06 mg ml-1。

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