Cloning and characterization of cDNAs preferentially expressed in the ovary of the mosquito, Anopheles gambiae.

摘要

Differential screening was used to isolate 2 expressed sequences from an ovarian cDNA library. These sequences are substantially enriched in ovaries of blood-fed female A. gambiae, as compared to the rest of the female, or male mosquitoes. One of these clones encodes an isoform of histone H2B, whose transcript is polyadenylated at the 3' end. The other cDNA clone encodes a protein which has been highly conserved in evolution and has been implicated in growth control, although its function is stillobscure. Both genes can be used to study gene activation during A. gambiae oogenesis. The GenBank accession number for the nucleotide sequences is X98185.