(Cloning, Characterization, and Expression of Genes/CDNA Encoding Lignin Peroxidases in Phanerochaete Chrysosporium, a White-Rot Basidiomycete): Progress Report, 1988-1989.

摘要

Progress has been made in 1988--1989 on a number of different research fronts focusing on the molecular biology of Phanerochaete chrysosporium. These include the cloning, sequencing and characterization of a gene that encodes the lignin peroxidase H2 protein, characterization of mutants in which lignin peroxidases are no longer subject to nitrogen regulation, and characterization of a lignin peroxidase (LIP) mutant. The latter mutants will be valuable tools for future studies on the genetics of lignin degradation system and for a better understanding of the physiological role of individual lignin peroxidase proteins in lignin degradation. We have also characterized a novel shuttle vector-based, non-integrative transformation system for P. chrysosporium which will very useful for conducting detailed molecular genetic studies on this organism.