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First report on in vivo fertility trial of frozen thawed boar semen in India

机译:印度冷冻解冻的公猪精液体内繁殖试验的第一份报告

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The present study was conducted to evaluate the in vivo fertility of frozen thawed boar semen. Twenty ejaculates collected from six mature boars were frozen in a programmable freezer. After freezing the semen was evaluated for different sperm quality parameters. Twenty five sows were inseminated artificially utilizing frozen thawed semen. The percentage of sperm motility, live sperm, live intact acrosome, plasma membrane intact sperm, HOST-reacted sperm, live sperm with high mitochondrial membrane potential, lipid peroxidised sperm and DNA-damaged sperm of frozen semen utilised for AI were 56.25 +/- 0.96, 63.75 +/- 1.47, 59.88 +/- 1.09, 41.08 +/- 1.01, 40.31 +/- 1.02, 86.23 +/- 1.29, 9.28 +/- 0.83 and 4.20 +/- 0.29 respectively. The farrowing rate was 44.00 per cent and the mean litter size at birth was 5.91 +/- 0.69. It could be concluded that the freezing and insemination protocol of boar semen used in the present study resulted in moderate fertility of frozen boar semen and it would help in further improvement and utilization of frozen boar semen for AI in India.
机译:进行本研究以评估冷冻解冻的公猪精液的体内繁殖力。从六个成熟公猪收集的二十种射精在可编程冰箱中冷冻。冷冻后,评估精液的不同精子质量参数。用冷冻解冻的精液对25头母猪进行人工授精。用于AI的精子活力,活精子,活完整顶体,质膜完好精子,HOST反应的精子,线粒体膜电位高的活精子,脂质过氧化精子和DNA损伤精子的百分比为56.25 +/-分别为0.96、63.75 +/- 1.47、59.88 +/- 1.09、41.08 +/- 1.01、40.31 +/- 1.02、86.23 +/- 1.29、9.28 +/- 0.83和4.20 +/- 0.29。产仔率为44.00%,出生时的平均产仔数为5.91 +/- 0.69。可以得出的结论是,本研究中使用的野猪精液的冷冻和授精方案导致了冷冻野猪精液的适度受精,这将有助于进一步改善和利用冷冻野猪精液用于印度的AI。

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