Thinner for freezing boar semen and method of freezing boar semen

摘要

the object of the application is diluent for freezing boar semen, which is a mixture of diluent u017cu00f3u0142tkowo - lactose of 89.5%, 9%, and glycerol in the amount of detergent based on laurylosiarczanu sodium concentration 1.5% and butylowanego hydroksytoluenu in quantities suitable the final concentration 1 mmol. the diluent u017cu00f3u0142tkowo - lactose contains 20% egg ovalbumin.the notification also includes the way of freezing semen. dense fraction of semen in the diluent to the semen storage at temperatures over 1:1, then cools to the temperature between 15 oc and ekwilibruje and spins.so obtained sperm suspension dispersible concentration 1.5 x l09 sperm / ml diluent u017cu00f3u0142tkowo - laktozowym and cooled to temperature 5u00b0c and ekwilibruje.then the sperm suspension dispersible diluent so as to obtain the concentration of 1 x l09 sperm / ml, and the final concentration of glycerol in the amount of 3%, after which the thus prepared semen filled straws of 0,5 ml and frozen in liquid nitrogen for temp pairs eraturze - 120u00b0c.