Effect of tris-buffer, egg yolk and glycerol on canine semen freezing

摘要

Currently, most researchers use Tris-buffer as an extender for canine semen preservation (Iguer-Ouada & Verstegen, 2000; Tsutsui et al., 2000). Tris preserves sperm energy by reducing fructolysis (Rodrigues, 1997). Egg yolk is often added to Tris asa cooling protector but there is a possibility of disease transmission by egg yolk, which creates an obstacle for the commercialization of semen between countries (Farstad, 1996). Glycerol is also used as an intracellular cryoprotector. Curry (2000) andHolt (2000) have however reported that glycerol has toxic effects on spermatozoa and the concentration of glycerol represents a balance between its toxic and protecting effects (England, 1993). Thus, it remains necessary to decrease risks to the spermatozoa and to facilitate a greater acceptance for the commercial use of frozen canine semen. This study aimed to test the effects of Tris-buffer, isolated or added only to egg yolk or glycerol, as an extender for canine semen freezing.