The gene expression of the Tvmfs transporter and defense enzyme activities from Trichoderma harzianum response to pH stress

摘要

The pH stress, one of the important environmental conditions, plays critical influence on microbial survival, colonization and ecology. It has been reported to be associated directly with its effects on microbial metabolisms, but there are few studies in literature. In current study that was investigated the impact of pH stress which is the major facilitator superfamily (MFS), a ubiquitous group of proteins involved in the transport of compounds produced by beneficial fungi, gene expression of?Trichoderma harzianum. Moreover, this experiment also determined the defense enzyme activities of?T. harzianum?response to the pH change. The results demonstrated that fungal growth, spore germination and biomass of?T. harzianum?were obviously inhibited at pH 3.0 and 10.0. The pattern ofTvmfs?gene expression was decreased in both pH conditions, and this suggested that this gene did functionally relate to pH stress response. Furthermore, the changes in enzyme activities including catalase and peroxidase were decreased, whereas kinase was increased with both pH stresses. Glucanase were decrease-changed as acidic and alkaline conditions. This experiment suggested that these key gene and enzymes help?T. harzianum?adapt for survival to pH stress, due to enhance or disrupt activity of the fungal metabolism. This is a valuable result to adjust the effective condition in growth of the beneficial fungi for biofertilizer in the near future.