哈茨木霉菌（Trichoderma harzianum）TR409诱导辣椒防御酶活性变化及防治疫病效果

摘要

On the basis of Phytophthora capsici Strain Pc-2 isolated, induction of defense enzymes in stem of Pepper, and control of Pepper Phytophthora blight by T. harzianum TR409 were analyzed. The results showed the enzyme activities in conidia-treated pepper reached their peaks earlier than those in chlamydospores treatment, however, the latter showed higher enzyme activity peaks in pepper. The maximum peaks of phenylalanine ammonialyase (PAL), peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) activities of pepper stem by chlamydospores-treated of TR409 were enhanced by 6.26, 2.33, 6.41 and 4.28 times over control. Treatments with conidiospores and chlamydospores of T. harzianum TR409 on pepper reduced the disease index of Pepper phytophthora blight from 59.5 to 29.4 and 27.6, respectively.%采用组织分离法获得辣椒疫霉菌(Phytophthora capsici)Pc-2,分析哈茨木霉菌(Trichoderma harzianum)TR409诱导辣椒茎基部防御酶活性变化及对辣椒疫病防治效果。结果表明：哈茨木霉菌TR409分生孢子和厚垣孢子处理后辣椒体内防御反应酶活性发生显著变化,厚垣孢子比分生孢子处理的酶活性高峰峰值更高,且出现的时间更晚,其中厚垣孢子灌根24h后接种辣椒疫霉菌的处理中辣椒体内苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)的活性分别比对照提高6.26,2.33,6.41和4.28倍。分生孢子和厚垣孢子处理后辣椒疫病的病情指数分别由对照的59.5降低至29.4和27.6。