14-3-3 genes are highly conserved,and they are involved in biotic and abiotic stress response as well as other cellular metabolic processes in soybean genome.Eighteen 14-3-3 genes had been identified and among them 16 genes could be transcribed.In this study,gene expressions of these 16 genes were assayed under phosphorus starvation in BX10 and TL1,tolerant-and sensitive-phosphorus deficiency cultivar respectively.Six 14-3-3 genes were differentially expressed in BX10 and TL1 under low phosphorus stress.Further analysis indicated that these 6 genes had interaction with soybean phosphate transporter PHT6,suggesting that soybean 14-3-3 proteins might have participated in phosphorus signal channel by interacting with PHT6.The results of this study could assist the application of 14-3-3 genes in soybean genetic improvement.%14-3-3是真核生物中一类高度保守的基因家族,参与生物和非生物胁迫中多种代谢途径的调控.在大豆基因组中已鉴定出18个14-3-3基因,其中16个可以转录.本文通过荧光定量PCR方法检测了这16个14-3-3基因在低磷胁迫下的响应,发现其中6个基因在耐低磷胁迫大豆品种BX10和不耐低磷胁迫大豆品种TL1中的表达存在差异.进一步分析表明,这6个基因的编码蛋白与大豆磷转运蛋白PHT6存在互作,表明大豆14-3-3蛋白可能通过与PHT6互作参与对磷信号通路的调控.本文结果将为在大豆中应用14-3-3基因培育耐低磷大豆品种提供参考.
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