研究了不同外植体及氮源浓度对甘草（Glycurrihiza uralensis）愈伤组织诱导的影响。在相同激素配比条件下,采用组织培养方法比较根、茎、子叶、下胚轴对愈伤组织诱导和生长的影响。结果表明,在不同外植体中,下胚轴愈伤组织平均诱导率最高,且出愈时间最早,褐化率最低,是形成愈伤组织的最佳材料。在特定的激素浓度条件下,不同浓度的氮源对下胚轴愈伤组织的诱导和生长有显著的影响,较低和较高浓度的氮源均不利于愈伤组织的生长,适宜于愈伤组织诱导和生长的最佳氮源浓度为60mmol.L-1。不同外植体和氮源浓度是影响甘草愈伤组织诱导和生长的重要因素。%An experiment was conducted to determine the effects of different explants and nitrogen concen tration on callus induction of Glycyrrhiza uralensis. The tissue culture method was used to compare the effects of root, stem, cotyledon and hypocotyls on callus induction and growth. The results of this study showed that the callus of hypocotyls was induced by higher induction rate, quicker induction time and low er browsing rate, indicating that the hypocotyls was optimal explants to induce the callus. The different concentration of nitrogen had the most significant effects on callus growth and induction of hypocotyls in certain hormone concentration, in which the lower and higher nitrogen concentration had negative effects on the growth of callus, and the optimal nitrogen concentration was 60 mmol·L^-1, which had the best effects on callus growth and induction. The different explants and nitrogen concentration were theimpor tant factors affecting the growth and induction of callus.
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