We studied embryo rescue and hybrid identification of three cross combinations (Chrysanthemum lavandulifolium×C. morifolium‘Die Ying’, C.chanetii×C.lavandulifolium, Ajania pacifica×Opisthopappus taihangensis) to select the inter-species hybrid between chrysanthemum efficiently .The offspring of these three combinations could only be obtained by em-bryo rescue rather than conventional cross breeding .The best time for embryo rescue was 16-24 days after pollination .MS medium supplemented with BA 1.5 mg/L and NAA 2.0 mg/L was the optimal medium for germination and growth .AFLP and morphological analysis proved the reliability of the hybrids .%为了提高菊花远缘杂交育种效率，对3个常规杂交育种方法难以获得杂种后代的组合：甘菊ב蝶影’、小红菊×甘菊以及矶菊×太行菊进行幼胚拯救，并对获得的杂种进行了AFLP 分子早期鉴定和形态学鉴定。结果表明，3个常规有性杂交难以或仅能获得较少杂种的杂交组合，通过幼胚拯救均可获得杂种后代，极大地提高了育种效率。较适宜的幼胚拯救时期为授粉后16～24 d；较适宜的培养基为MS＋BA1．5 mg・ L-1＋NAA2．0 mg・ L-1；AFLP及形态学鉴定证实了杂种的真实性。
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