Processes for preparing sterile male plants, for restoring fertility in a sterile male plant, for producing a homozygous sterile male lineage, and for the production of fertile hybrid plants, fertile hybrid plants, vector, agrobacterium strain, plants transformed with the strain of agrobacterium, sterile male, and homozygous sterile male lineage

摘要

"PROCESSES FOR PREPARING STERILE MALE PLANTS, FOR FERTILITY RESTORATION ON A STERILE MALE PLANT, TO PRODUCE A HOMOZYGOTIC STERILE MALE LINE, AND FOR THE PRODUCTION OF HYDRATED, HERITAGE, PLANTED HERITAGE PLANTS, PLANTED PLANTS CEPA DE AGROBACTERIUM, MALE STERILE, AND MALE LINE STERILE HOMOZIGOTIC ". The present invention is directed to the production of sterile male plants by endowing them with recombinant DNA capable of specific expression, in the male reproductive system of a plant, of the enzyme trehalose phosphate phosphatase (TPP). Restoration of fertility can be established by endowing said sterile male plants with recombinant DNA capable of expression of trehalose phosphate synthase (TPS) under the control of an inducible promoter or with recombinant DNA capable of expressing a suppressor protein that suppresses the expression of TPP under the control of an inducible promoter. This (s) possibility of inducible restoration allows (s) the maintenance of a homozygous sterile male lineage. Restoration can also be performed by spraying sterile male plants with gibberellinic acid. For the production of hybrids or hybrid seeds, a site-specific recombination system is provided by inserting two site-specific recombination sites flanking the recombinant DNA encoding for TPP and crossing the sterile male lines with lines that express the corresponding recombinase. Crossing the recombinase, the gene encoding TPP is excised and fertile hybrids are produced. site-specific flanking the recombinant DNA encoding TPP and crossing the male sterile lines with lines that express the corresponding recombinase. Crossing the recombinase, the gene encoding TPP is excised and fertile hybrids are produced.