野禽新城疫病毒F和HN基因的遗传变异趋势

摘要

Newcastle disease virus carried by wildfowl is the important reason and potential source which caused Newcastle disease in poultry.Based on 44 wildfowls' (including wild duck, parrot, pigeon, swan ,mandarin duck, wild goose, turkey, etc.) NDV from genbank and the pigeon NDV (PB9601) and the penguin NDV(QE01) cloned and sequenced by our laboratory ,the F gene cleavage site sequence, genotype and the hereditary variation of the deduced amino acids of HN and F gene were analyzed.The results showed the most NDV isolates genotype belonged to Ⅶ and Ⅵ and the others existed different genotypes (I, II, V, IX).Through the hereditary variation analysis of F gene,the isolates had obvious regional and genus traits among the same species or the same regions; most of the isolated strains had farther genetic distance with LaSota, V4, B1, TEX-48.The similar results can be obtained through the genetic evolution of relations of HN genes.%野禽携带的新城疫病毒是新城疫流行传播的重要的潜在传染源.结合本实验室克隆测序的鸽NDV(PB9601)、企鹅NDV(QE01)以及GenBank下载的44株野禽(包括野鸭、鹦鹉、鸽子、天鹅、鸳鸯、雁、火鸡等)的NDV毒株的融合蛋白(Fusion gene,F)基因和血凝素蛋白(Hemagglutinin-neuraminidase gene,HN)基因分别进行了F蛋白裂解位点、基因分型以及F和HN基因氨基酸全长的遗传距离分析.结果表明:野禽感染NDV F基因的基因型以Ⅶ和Ⅵ为主,同时I、II、V和IX多种基因型并存.同一种属、同一地区的毒株间的遗传距离较近,具有明显的种属性和区域性,且绝大多数分离株与经典毒株LaSota、V4、B1、TEX-48等的遗传距离相对较远;对HN基因的遗传进化关系分析得出了相似的结论.