金针菇工厂化生产的液体菌种开发与应用

摘要

[目的]开发金针菇工厂化生产所需的液体菌种.[方法]从工厂化生产的菌株与二级液体菌种培养基的筛选,液体菌种培养条件的优化及金针菇液体菌种在工厂化生产的应用试验等方面进行研究.[结果]在4个金针菇工厂化生产菌株中,金针菇工厂化生产的液体菌种开发宜以F白"18"为出发菌株;二级液体菌种的培养基宜为马铃薯15%、麸皮 7%、蔗糖 2%、 磷酸二氢钾 0.1%、 硫酸镁0.05%;培养条件为接种量10%,转速150 r/min,温度23 ℃,pH自然,发酵罐培养时间结束点和适宜的菌龄均为144 h;在工厂化生产应用中,液体菌种较固体菌种发菌速度快,生产周期缩短9 d,生物转化率提高了6.38百分点.[结论]该研究可为金针菇工厂化生产所需液体菌种的开发提供技术方法.%[Objective]To explore liquid fungus suitablely ultilized in the process of Flammulina velutipes industrial production.[Method]We screened different strains of F.velutipes for factory production and the second-class liquid culture media suitable for its liquid fungus,optimized its culture conditions and carried out application trials on the use of F.velutipes liquid fungus for industrial production.[Result]The F bai"18" strain was the most suitable liquid fungus for production of F.velutipes,the optimum formula of liquid fungus culture medium was potato 15%,bran powder 7%,sucrose 2%,KH2PO4 0.1%,MgSO4 0.05%,and the optimum fermentation culture condition was as follows:intial pH naturally,liquid spawn quantity of 10%,rotation speed 150 r/min,temperature 23 ℃,culture time 144 h in fermentor,suitable cell age 144 h.In the test of industrial cultivation of liquid and solid fungus of F bai"18",its mycelia of liquid fungus has grown faster than that of solid fungus,resulting in reduction of its production period by 7 days and increasement of its biological conversion rate by 6.38 percent points.[Conclusion]The research can provide technical method for the development of liquid fugus of F.velutipes industrial production.