H9N2亚型禽流感病毒单克隆抗体的制备及其表位鉴定

摘要

[Objective] To prepare H9N2 subtype avian influenza virus antibodies and identify epitope.[Method] The epitopes of two monoclonal antibodies (McAbs) specific to H9 subtype avian influenza virus (AIV) were identified by cell fusion with the BALB/c mice immunized with purified H9N2 AIV WD-1.[Result]The 4C10 and 6E3 were belonged to IgG 1 and IgG 2b,repectively.The ELISA titers were 1∶103 and 1∶105.The HI titers in their ascites were 215 and 214.Two monoclonal antibodies had chicken embryo neutralizing activity.The antigen epitopes of two monoclonal antibodies were all linera epitopes targeting HA protein.[Conclusion] The study laid the foundation for the establishment of rapid diagnostic method of avian influenza virus.%[目的]制备H9N2亚型禽流感病毒单克隆抗体和鉴定表位.[方法]选取H9N2亚型禽流感病毒(AIV)WD-1株的纯化抗原免疫BALB/c小鼠,对获得2株抗H9N2亚型禽流感病毒的特异性单抗4C10和6E3表位进行鉴定.[结果]单抗4C10和6E3分别属于IgG1和IgG2b亚型,ELISA检测效价分别为1∶103和1∶105;HI效价分别为215和214;2株单抗均具有鸡胚中和活性.利用噬菌体展示表位技术对2株单抗的抗原表位进行鉴定,结果显示均为针对HA 蛋白的1个线性表位.[结论]该研究为禽流感病毒快速诊断方法的建立提供了依据.