Aim To evaluate the protective effects of XCG-7. an anticomplementary polyphenol from the seed of Toona sinen.si.s( Meliaceae ) on complement-injured SH-SY5Y cells. Methods A model of neuronal injury was established on SH-SY5Y cells through activation of complement by cobra venom factor, an alternative pathway-specific activator. SH-SY5Y cells were exposed to different concentrations of XCG -7 . and then the release of lactate dehydrogenase and cell viability were measured at 24 h and 48 h. and the activity of caspase-3/7 was determined at 24 h. Results The anticomplementary polyphenol XCG-7 promoted SHSY5Y cell proliferation. The polyphenol inhibited the reduction of cell viability induced by activated complement , and moderated the release of lactate dehydrogenase . and decreased the apoptosis in the cells. Conclusion The anticomplementary polyphenol XCG-7 from the seed of Toona .smensis can promote SH-SY5Y cell growth, and protect SH-SY5Y cells from complementinduced injury.%目的 评价香椿果抗补体活性多酚XCG-7对补体损伤神经细胞的保护作用.方法 采用眼镜蛇毒因子特异激活血清补体,诱导人神经母细胞瘤细胞SH-SY5Y损伤.通过测定乳酸脱氢酶释放量、细胞活力和Caspase-3/7活性等指标,评价XCG-7对补体损伤神经细胞的保护作用.结果 XCG-7对SH-SY5Y细胞的生长具有促进作用,明显抑制补体损伤导致的细胞活力下降,在一定程度上减轻乳酸脱氢酶的释放,抑制细胞凋亡的发生.结论 香椿果抗补体活性多酚XCG-7能促进SH-SY5Y细胞的生长,对补体损伤神经细胞具有一定的保护作用.
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