反义Ku70在人肺癌细胞的表达及对放射线的敏感性研究

摘要

Objective:To study the expression of the antisen se Ku70 in a human's lung carcinoma cell line(LCA) and its high-radiosensitivit y.Methods:The expression of the Ku70 protein in the Ku70- LCA and its radiosensitivity was detected by Western-blott ing and Cell Radiosensitivity Taste (to see The Cell Clonogenic Assay and Surviv al Curves).Results:(1)Only the sense Ku70 protein was defe cted in the Ku70-LCA,but the sense Ku70 protein wasn't defected in the Ku70as -LCA by Western-blotting.(2)The radiosensitivity was increased in the Ku70as- LCA and their radiosensitivity depend on the radiating dose(P＜0.01).Conclusion:After the expression of sense Ku70 protein in t he Ku70as-LCA was blocked by antisense Ku70,the Ku70as-LCA should be high-rad iosensitivit y,the Ku70 is a candiate target for cancer gene therapy.It was the first step fo r the study of tumor's specific radiating and gene therapy in the future.%目的：研究反义Ku70在人肺癌细胞的表达及对放射线的超敏效应。方法：采用Western-boltting法证明，Ku70-LCA中Ku70蛋白的表达情况，采用体外放射线敏感实 验证明，反义Ku70 在人肺癌细胞的表达及对放射线的超敏效应，其结果以细胞存活克隆率和剂量效应曲线表示 。结果：(1)对照组和导入正义Ku70基因的LCA组细胞可见Ku70蛋白表达印 迹，导入反义Ku70基因LCA组细胞未见Ku70蛋白表达印迹。(2)导入反义Ku70基因的LCA组 细胞对放射线的敏感性明显增强(P＜0.01)，并呈剂量依赖性。结论：反义Ku70封闭了肿瘤细胞的正义Ku70蛋白表达后，对放射线呈超 敏效应，为提高肿瘤放疗的特异性和靶向基因治疗奠定了基础。