乌司他丁对缺血再灌注肺组织细胞间黏附分子-1和P-选择素基因mRNA表达的影响

摘要

OBJECTIVE To explore the effects of ulinastatin ( UTI ) on the mRNA expression of intercellular adhesion molecule ( ICAM ) - 1 and P - selection in pulmonary tissue after ischemia reperfusion. METHODS Eighteen New Zealand rabbits were randomly divided into 3 groups of 6 each: low potassium dextran ( LPD ) group, UTI group and control group. In vivo lung ischemia reperfusion model was established in rabbit, after hilum of left lung was clamped, the left lower lung lobe was stored at 10℃ in a special made lung preservation container for 2 hours and reperfused for another 2 hours. In LPD group and UTI group the left lower lobe was perfused with LPD solution or LPD solution contained ulinastatin respectively, without anything perfused in control group. The pulmonary tissue was obtained at three time intervals and the mRNA expression of ICAM - 1 and P - selection in pulmonary tissue was measured using RT - PCR technique. RESULTS The mRNA expression of ICAM - 1 at 2 hours after ischemia and 2 hours after perfusion in UTI group was significantly lower than that in LPD group and control group. The P - selection mRNA expression at 2 hours after perfusion in LPD group and control group was significantly higher than pre - ischemia and 2 hours after clamping hilum of left lung.There was no such significant difference in UTI group. CONCLUSION UTI can inhibit the upregulation of the mRNA expression of ICAM -1 and P -selection after ischemia reperfusion in pulmonary tissue, so it is profitable for pulmonary protection.%目的 探讨乌司他丁(UTI)对在体缺血再灌注后肺组织细胞间黏附分子(ICAM)-1和P-选择素基因mRNA表达的影响.方法 新西兰兔18只随机分为3组:低钾右旋糖酐液(LPD)组、UTI组和对照组.建立兔在体肺缺血冷存再灌注模型,阻断左肺门后将左肺下叶在体冷存于10℃肺保存器内2 h,再灌注2 h.LPD组灌注LPD液,UTI组灌注含UTI的LPD液,对照组不灌注肺保护液.分别于左肺门阻断前、缺血2 h、再灌注2 h取左肺组织,以RT-PCR技术检测ICAM-1和P-选择素基因mRNA表达.结果 缺血2 h及再灌注2 h,UTI组ICAM-1基因mRNA表达量显著低于LPD组和对照组;再灌注2 h,LPD组和对照组P-选择素基因mRNA的表达量明显高于缺血前和缺血2 h,UTI组则无明显变化.结论 乌司他丁可抑制缺血冷存再灌注后肺组织ICAM-1和P-选择素基因mRNA表达上调,有利于肺保护.