目的：采用高效液相色谱梯度洗脱法测定三七总皂苷中人参皂苷 Rb1、人参皂苷 Rg1和三七皂苷 R1的含量。方法色谱柱为 C18柱（250 mm ×4.6 mm，3.5μm），流动相为乙腈-水、梯度洗脱，检测波长为203nm，流速1.0 mL / min，柱温为室温。结果人参皂苷 Rg1进样量线性范围是6.0~18μg（ r =0.9967），平均回收率为98.79%，RSD =0.33%（ n =6）；人参皂苷 Rb1进样量线性范围是6.0~18μg （ r =0.9964），平均回收率为98.64%，RSD =0.46%（ n =6）；三七皂苷 R1进样量线性范围是1.6~4.8μg（ r =0.9971），平均回收率为98.84%，RSD =0.56%（ n =6）。结论该方法准确、灵敏度高，可用于三七总皂苷的质量控制。%Objective To establish a HPLC gradient elution method for the determination of ginsenoside Rb1,ginsenoside Rg1and notoginsenoside R1 in Panax notoginseng saponins. Methods The HPLC method was carried out on the C18 column(250 mm × 4. 6 mm, 3. 5 μm) with acetonitrile - water as the mobile phase. The detection wavelength was 203 nm and the flow rate was 1. 0 mL / min.Results The linear range of the ginsenoside Rg1 sample size was 6. 0 - 18 μg( r = 0. 996 7),the average recovery rate was 98. 79% ,RSD = 0. 33%( n = 6). The linear range of ginsenoside Rb1 sample size was 6. 0 - 18 μg( r = 0. 996 4),the average recovery rate was 98. 64% ,RSD = 0. 46%( n = 6). The linear range of notoginsenoside R1 sample size was 1. 6 - 4. 8 μg( r = 0. 997 1),the average recovery rate was 98. 84% ,RSD = 0. 56%( n = 6). Conclusion This method is accurate and highly sensitive,and can be used to control the quality of Panax notoginseng saponins. Key words:panax notoginseng saponins.
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