巨大芽胞杆菌与普通生酮基古龙酸菌共培养过程中相互作用分子机制研究进展

摘要

混菌发酵法广泛应用于维生素C前体2-酮基-L-古龙酸的生产.为进一步改善工艺,多年来,科研人员一直致力于研究发酵过程中两菌相互作用的科学本质.目前,随着组学技术、高通量技术、生物信息学和生理学等多种技术与学科的迅速发展,为深入研究相互作用分子机制提供了新的方法和工具.通过蛋白质组学、代谢组学、比较基因组学、转录组学等多种组学数据的挖掘和分析,提供了系统中各层次之间的相互作用关系网络.在此基础上结合高通量的生理学验证分析,为诠释两菌相互作用分子机制和开发代谢工程改造策略奠定了基础.本文就近些年来在该研究方向的进展及其应用进行了简要归纳,并提出进一步研究的方向.%Co-culturing Bacillus megaterium and Ketogulonigenium vulgare is widely applied to 2-keto-gulonic acid production.For optimizing the process, numerous researchers studied on the symbiotic molecular mechanism of the co-culture process.The research was promoted greatly owing to omics technologies, bioinformatics, high throughput technologies and physiology.Recently, the proteomic, metabolomic, comparative genomics and transcriptomics were performed to the research.These omics data provided us the interaction network of the artificial ecosystem in multilevel.Combining with the physiological validation based on the high throughput method, we can elucidate the molecular mechanism in detail, which will facilitate us to develop strategies for metabolic engineering.The paper reviewed the recent developments of symbiotic molecular mechanism research in this co-culture process and its applications.In addition, we proposed the future research needs.