Simultaneous HPTLC Analysis of E-Guggulsterone, Z-Guggulsterone,11-Keto-p-Boswellic Acid, and 3-Acetyl 11-Keto-p-Boswellic Acid in an Anti-Arthritic Formulation

摘要

A simple, precise, and rapid HPTLC method has been established for simultaneous analysis of E-guggulsterone, Z-guggulsterone, 11-keto-P-boswellic acid (11-KBA), and 3-acetyl-11-keto-P-boswellic acid (A-11-KBA) in an anti-arthritic formulation. The method involves densitometric evaluation of E and Z-guggulsterone, 11-KBA and A-11-KBA after their resolution on pre-coated silica gel 60F_254 plates with n-hexane-chloroform-ethyl acetate-methanol 10:3:3:1 (v/v) as mobile phase. Detection was at 254 nm. Good resolution was achieved, with R_F 0.61 +- 0.03,0.68 +- 0.03,0.28 +- 0.03, and 0.39 +- 0.03 for E and Z-guggulsterone 11-KBA, and A-11-KBA, respectively. The method was validated for specificity, linearity, precision, accuracy, and robustness. The method was linear in the range 10-90 ng per band for E and Z-guggulsterone and 50-450 ng per band for 11-KBA and A-ll-KBA. Recovery at 80, 100, and 120% was 96.93 +- 0.27, 97.39 +- 0.22,97.60 +- 0.61, and 97.19 +- 0.41% for E- and Z-guggulsterone, 11-KBA, and A-ll-KBA, respectively. The method is simple, specific, precise, accurate, reproducible, and gives results comparable with those from an HPLC method.