酶法提取稻谷加工副产物碎米中蛋白成分工艺的研究

摘要

通过单因素实验和正交实验,用中性蛋白酶水解法对稻谷加工副产物碎米进行了蛋白质的提取研究,得到如下最佳提取条件:酶解时间2.5h、酶解温度55℃、酶的添加量1.0％ (w/w)、液固比10∶1.在此条件下蛋白质的提取率可达74.8％.%The technology of extracting protein from cereal processing by - product broken rice with neutral protease hydrolysis method was investigated through single - factor experiment and orthogonal experiment. The optimum extraction conditions were acquired as follows; enzymolysis time 2. 5 h, enzymolysis temperature 55℃, enzyme addition amount 1.0% ( w/w) and the ratio of liquid to solid 10:1. Under the above optimal conditions, the protein extraction rate reached 74.8%.