Enzymatic extraction process in an aqueous medium oil and protein from plant material

摘要

Procedure enzymatic extraction in aqueous medium of oils, proteins and fermentable sugars from plant material, comprising the following steps: a) adding water to the plant material having a suitable particle size, b) addition of an enzyme mixture contains at least one cellulase, at least one hemicellulase and at least one peptinasa, the relationship between the activity of pectinase and cellulase activity of between 0.3 and 2.5, and more preferably between 0.35 and 0.45 being and the ratio being comprised peptinasa activity and activity of hemicellulase between 1.10-2 and 0.5, and more preferably between 1.10-2 and 2.10-2, with lower activity peptinasa 120 .mu.mol / min / ml, and preferably less than 100 .mu.mol / min / ml, wherein the amount of enzyme mixture is between 0.25% and 10% by volume of water / vegetal material mixture, preferably between 1% and 6%, c) incubation b garlic agitation of the plant material and the enzyme mixture to release in the medium oils reaction, proteins and fermentable sugars, for a duration which depends on the desired yields, d) separation of the reaction medium to obtain free oil, an aqueous phase contains proteins and fermentable sugars, and a solid phase, e) optionally separation of an emulsion free oil or aqueous phase, and recycling of emulsion in the reaction medium, f) separation of proteins and fermentable sugars the aqueous phase.