目的:探讨辛伐他汀对哮喘气道重构小鼠肺组织转化生长因子-β1 (TGF-β1) 表达的影响.方法: 采用随机分组的方法, 将60只雌性SPF级BALB/C小鼠分为哮喘组、辛伐他汀组、对照组, 每组20只. 观察和比较各组小鼠肺组织和气管的病理改变, 同时采用图像分析软件测量各组小鼠肺组织中支气管壁面积 (WAi) 、平滑肌层面积 (WAm) 和支气管基底膜周长 (Pbm); 使用Real time PCR和Western blot方法分别检测小鼠肺组织TGF-β1 mRNA和TGF-β1蛋白表达.结果: 哮喘组小鼠肺组织病理改变程度最严重, 辛伐他汀组病理改变程度虽高于对照组, 但较哮喘组减轻; 哮喘组小鼠支气管壁厚度及平滑肌层厚度高于辛伐他汀组和对照组; 哮喘组小鼠肺组织TGF-β1mRNA水平高于辛伐他汀组和对照组, 哮喘组小鼠肺组织TGF-β1蛋白水平高于辛伐他汀组和对照组, 差异均有统计学意义. 结论:辛伐他汀可能通过减少肺组织TGF-β1表达而抑制哮喘气道重构.%Objective: To observe the effects of simvastatin on transforming growth factor-β1 (TGF-β1) expression in the lung tissue of asthmatic mice with airway remodeling. Methods: A total of 60 SPF grade female BALB/C mice were randomized into groups of asthma, simvastatin administration, and control (n = 20 for each group). Pathological changes of the lung tissues and airways were observed and compared among groups of mice. Image analysis software was used to measure the internal wall area (WAi), smooth muscle area (WAm) and the perimeter of basement membrane (Pbm) in bronchial lung tissues, and real time PCR and Western blot were used to determine the expression levels of TGF-β1mRNA and protein in the lung tissues. Results: Pathological change was the severest in mice in asthma group. Although mice in the simvastatin administration group had severer pathological change than the controls, yet the change was minor compared to mice in the asthma group. Mice in the asthma group had thicker bronchial wall and smooth muscle layer as well as higher TGF-β1mRNA expression and protein level in the lung tissues than those in control group. The difference was statistically significant. Conclusion: Simvastatin may inhibit airway remodeling through reducing TGF-β1 expression in the lung tissues.
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