Effects of Radix Piatycodonis Polysaccharide on Expressions of IFN-γ, IL-4 and IL-5 in the Bronchoalveolar Lavage Fluid and NF-kB in the Lung Tissue of Asthmatic Rats

摘要

To investigate the effect of Radix playtycodoni polysaccharide (RPPS) on rats with ovalbumin-induced asthma and its mechanisms. Methods: 48 Wistar rats were randomly divided into blank control group, asthma model group, dexamethasone control group (1 mg / kg), and high-dose RPPS group (40 mg / kg), moderate-dose RPPS group (20 mg / kg) and low-dose RPPS group (10 mg / kg). The ovalbumin sensitization method was used to establish the asthma model in rats. The rats were given it in the atomization administration for two weeks and then were sacrificed. The BALF was collected for the count of eosinophils (EOS); enzyme-linked immunosorbent assay (EL1SA) method was employed for the determination of 1L-4, IL-5, and IFN-γ levels in BALF; the expression of NF-kB in the lung tissues was measured by Western blot.