Effects of Plant Growth Regulators on Callus Proliferation of Chinese Kale

摘要

Endogenous and exogenous plant growth regulators play essential role in the growth and proliferation of calluses. To establish an efficient protocol of callus proliferation, various concentrations of a-naphthaleneacetic acid (NAA) (0.4, 0.5, 0.6 mgL~(-1)) in combination with 6-benzylaminopurine (6-BA) (1.5, 2.0, 2.5 mgL~(-1)) on callus proliferation of Chinese kale were evaluated. The results showed that the best callus proliferation was observed on MS medium with 0.4 mgL~(- 1) NAA and 2.5 mgL~(-1) 6-BA after 30 d of culture, and the morphology of the callus was yellowish in colour and friable. This study provides a basis for future studies on genetic improvement for Chinese kale.