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INDUCTION OF CALLUS OF LIMONIUM SINUATUM, CULTURE AND PROLIFERATION OF CALLUS AND CREATION OF REDIFFERENTIATED PLANT BODY
INDUCTION OF CALLUS OF LIMONIUM SINUATUM, CULTURE AND PROLIFERATION OF CALLUS AND CREATION OF REDIFFERENTIATED PLANT BODY
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机译:柠檬草愈伤组织的诱导,愈伤组织的培养和繁殖以及减化植物体的建立
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摘要
PROBLEM TO BE SOLVED: To carry out high frequency mutation breeding, cell fusion breeding, etc., with other related genera by culturing a part of a plant such as Limonium sinuatum in a culture medium containing picloram. ;SOLUTION: Induction of callus is carried out by culturing a part of leaf cut piece, etc., of a plant body of Limonium sinuatum in a culture medium containing picloram (preferably having 0.1-1.0 mg/L concentration in the culture medium). Furthermore, callus culture and proliferation of Limonium sinuatum is preferably carried out by culturing callus in a culture medium containing picloram and redifferentiated plant body of Limonium sinuatum is preferably created by culturing induced callus or cultured and proliferated callus in a culture medium containing cytokinin (preferably having 0.1-1.0 mg/L concentration in the culture medium) such as benzyladenine or zeatin.;COPYRIGHT: (C)2000,JPO
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