Acridine orange staining for cell analysis

摘要

This work demonstrated the potential use of an acridine orange (AO) dye for nuclear to cytoplasmic (N/C) ratio analysis. The interaction of the AO dye to DNA and RNA emitted the green fluorescence in the cell nucleuses and red fluorescence around the cytoplasmic area by using a 488 nm excitation light source. Average of green to red fluorescence ratio of the HUF and Caski cells could represent an approximate N/C ratio which is calculated by semi-automated cells analysis software. Results from this study demonstrated the possibility of using the low cost AO dye to indirectly measure the N/C ratio in cell for abnormal cell diagnosis and classification.