RAPID DETERMINATION METHOD OF APOPTOTIC CELLS IN LARGE QUANTITY SAMPLES USING ACRIDINE ORANGE DYE STAINING METHOD

摘要

Purpose: providing a measuring method of the cell of the apoptosis using an acridine orange dye decoration method, to quickly and easily detect the cell of apoptosis, handles a large amount of samples in a short time. Construction: the measuring method by using the cell of the apoptosis of the irradiation of an acridine orange dye decoration method includes the following steps:: irradiating the peripheral blood cell with gamma ray; It is centrifuged after illumination and cultivates lymph corpuscle; It handles the lymph corpuscle manually cultivated according to one fragment-fixing method and prepares and use processed lymphocytic sliding; Contaminate the unit in the sliding with acridine orange dye; Pass through the cell of the apoptosis of a micro- sem observation dye.