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Development and characteristics of a new picosecond fluorescence lifetime system

机译:新型皮秒荧光寿命系统的开发和特征

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A new time-resolved fluorescence measurement system has been developed. A compact streakscope is used as a detector, and the combination of a spectrograph and a streakscope enables to measure the fluorescence lifetime and spectrum simultaneously using single photon counting method with a time resolution of 15ps (5ps with fitting software ). The dynamic range is achieved up to 105. The instruments response function (IRF) of a streak scope is nearly Gaussian, and it has no tail and no "after pulses" which are observed at the IRF of a photomultiplier used in the time correlated single photon counting system (TCPC). So, a short lifetime of several ten picoseconds can be measured with accuracy. In addition, two dimensional (time and wavelength) single photon counting can be performed without scanning the wavelength, so time resolved spectrum can be measured in a short time with a high S/N ratio.
机译:已经开发了一种新的时间分辨荧光测量系统。紧凑的晶片用作检测器,并且光谱仪和晶片的组合使得能够使用单光子计数方法同时测量荧光寿命和光谱,其时间分辨率为15ps(5ps,配合软件)。动态范围最多可实现105.条纹范围的仪器响应函数(IRF)几乎是高斯,并且它没有尾部和脉冲之后,在时间相关的光电倍增管的IRF处观察到的脉冲。光子计数系统(TCPC)。因此,可以通过精度来测量几十个皮秒的短寿命。另外,可以在不扫描波长的情况下执行二维(时间和波长)单光子计数,因此可以在具有高S / N比的短时间内测量时间分辨频谱。

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