Low-Wavenumber Raman and Synchrotron Studies of Proteins and Protein/Water Interactions. From Model System to Animal and Human Skin

摘要

Water is the most abundant substance in human cells. Water accounts for about 70% of a cell's weight. Water is important for protein dynamics and function in living cells. However, the role of water in bio-systems is far from well understood. In this context hydrogen bonding is certainly an important aspect. Vibrational spectroscopy is an important tool in studies of bio-molecular hydrogen bonding. For this purpose the NH and OH-stretching regions are most often used, but the stretching bands are only slightly perturbed by changes in the hydrogen bonds. The low wavenumber spectrum from around 50 to 300 cm"' yields more direct information about the hydrogen bonds'.