The establishment of detecting alpha fetoprotein(AFP) with immunofluorescence chromatography

摘要

This study was designed to develop a immunofluorescence chromatography kit for detection of AFP in human serum, and to evaluate the method performance. The fluorescent polystyrene particle conjugated with the monoclonal antibody MIC09 was used as a fluorescent label. The monoclonal antibody MIC10 and mouse anti-rabbit IgG were immobilized on the nitrocellulose membrane as the test line and control line. The linear response rang of the method to detect the serum AFP was 0.34-500ng/ml. And the intra-and inter-assay coefficient were 5.3% and 8.14%. Total 1200 samples were tested by the immunofluorescence chromatography kit, and the overall coincidence rate was 93.18%. The positive coincidence rate was 94.56%, the negative coincidence rate was 91.47%, and the Kappa value surpassed 0.8, which indicated that there was no significant difference between the results of the immunofluorescence chromatography kit and Elisa kit. In conclusion, the immunofluorescence chromatography kit for assaying AFP provided high sensitivity, rapid, simple operation, low cost, single-servings and can be extensively used for clinical operations.