Methods for Producing Fused Lipid Nanoparticles and Fused Lipid Nanoparticles for Target Cell-Specific Production of Therapeutic Proteins and for the Treatment of Target Cell-Related Diseases, Diseases, or Disorders And how to use

摘要

Target cell-specific production of therapeutic proteins, such as pro-apoptotic proteins, within target cells, including target cells associated with aging, disease, or other condition, particularly target cells that are aging or cancer cells. Nucleic acid-based expression constructs for. Formulations and systems, including fused lipid nanoparticles (LNP) formulations and systems, and aging cells and / or cancer cells associated with aging, disease, or other conditions, for delivery of nucleic acid-based expression constructs. Methods for producing and using such nucleic acid-based expression constructs, compositions, and systems to reduce, prevent, and / or eliminate cell growth and / or survival, as well as aging, disease, etc. A fused LNP containing an expression construct for target cell-specific production of a therapeutic protein, such as an antiproliferative protein, in target cells associated with or other conditions, particularly target cells such as senescent cells or cancer cells. Methods for the treatment of aging, disease, or other conditions via in vivo administration of a formulation, such as a formulation, are also provided. [Selection diagram] Fig. 1