PRIMARY LIVER CANCER CELL CULTURE MEDIUM, PRIMARY LIVER CANCER CELL CULTURING METHOD AND APPLICATION THEREOF

摘要

Provided are a primary liver cancer cell culture medium and a primary liver cancer cell culturing method for quickly and stably culturing primary liver cancer cells. The foregoing primary liver cancer cell culture medium comprises glutamine, non-essential amino acids, a basic fibroblast growth factor, a hepatocyte growth factor, IL-6, an epidermal growth factor, insulin, Y27632, an N2 additive, a B27 additive, Primocin, penicillin and streptomycin, fetal bovine serum, optionally hydrocortisone, and optionally R-spondin1. A cell model obtained from the primary liver cancer cell culture medium and primary liver cancer cell culturing method can be used in the efficacy evaluation and screening of drugs.