COLORECTAL CANCER SOLID TUMOUR PRIMARY CELL AND COLORECTAL CANCER ASCITIC FLUID PRIMARY TUMOUR CELL CULTURING METHOD, AND MATCHING REAGENT

摘要

The present invention discloses a method for culturing colorectal cancer solid tumor primary cells and colorectal cancer ascites primary tumor cells and supporting reagents. The present invention provides a method for culturing colorectal cancer solid tumor primary cells and colorectal cancer ascites primary tumor cells and supporting reagents. The core of this technology is: (1) colorectal cancer solid tumor tissues are treated with mild cell dissociation reagents, and colorectal cancer cells are isolated from ascites with a mild method, thereby ensuring the vitality of cancer cells to the greatest extent; and (2) a special serum-free medium is prepared, and colorectal cancer solid tumor-derived tumor cells are cultured in vitro with a suspension culture system to ensure normal expansion of the cancer cells while eliminating the interference of normal cells to the greatest extent. The colorectal cancer primary cell culture obtained by the method of the present invention can be used for in vitro experiments, second-generation sequencing, building of animal models, and building of cell lines at multiple cell levels. It is foreseeable this culture method has broad application prospects in the fields of research and clinical diagnosis and treatment of colorectal cancer.