CARRIER FOR CULTURING PRIMARY CANCEROUS CELL AND METHOD FOR CULTURING PRIMARY CANCEROUS CELL USING THE SAME

摘要

PURPOSE: To obtain a carrier, containing a specific compound, having specified properties, capable of remarkably improving the proliferation and survival ratio of a primary cancerous cell and/or a mass thereof directly collected from a patient suffering from cancer and enabling the culture, subculture and recovery without damaging, etc., the cell. ;CONSTITUTION: The carrier comprises a polymeric compound having a hydrophobic and a hydrophilic parts in the molecule and 0 to ≤60°C sol-gel transformation temperature and is capable of reversibly manifesting a liquid state (gel state) at a lower temperature than the transformation temperature. Furthermore, this carrier is mixed with a culture medium at a lower temperature than the sol-gel transformation temperature and a primary cancerous cell or a mass thereof or both are then mixed in the resultant mixture to provide the gel state at a higher temperature than the sol-gel transformation temperature. Thereby, the culturing is carried out.;COPYRIGHT: (C)1994,JPO&Japio