INTEGRATED CAPTURE AND AMPLIFICATION OF TARGET NUCLEIC ACID FOR SEQUENCING

摘要

There is described a method of preparing a target nucleic acid, comprising contacting a target nucleic acid with a capture probe and an immobilized probe, the capture probe comprising a first segment that binds to the target nucleic acid and a second segment that binds to the immobilized probe, wherein the target nucleic acid binds to the first segment of the capture probe, and the second segment of the capture probe binds to the immobilized probe, thereby forming a capture hybrid capturing the target nucleic acid; and performing a PCR amplification with real time detection of the captured target nucleic acid without a step of dissociating the capture hybrid before initiating thermocycling in the PCR amplification; wherein the PCR amplification is performed in the same vessel as the contacting step.