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METHOD FOR ENZYMATICALLY ASSAYING CREATININE OR THE AMMONIUM ION AND REAGENT KIT FOR CARRYING OUT SAID METHOD
METHOD FOR ENZYMATICALLY ASSAYING CREATININE OR THE AMMONIUM ION AND REAGENT KIT FOR CARRYING OUT SAID METHOD
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机译:酶法测定肌酐或铵离子及试剂盒的方法
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摘要
A method for enzymatically assaying creatinine consists in determining the quantity of ammonium ion released by creatinine. The method is characterized in that the assay is performed in the presence of a hydroxamic acid or a salt thereof. The invention also concerns a method for eliminating errors in enzymatic assays of the ammonium ion consisting in introducing in the reactive medium, with the sample, a hydroxamic acid or salt thereof. A set of reagents is also provided for enzymatically assaying creatinine and comprises glutamate dehydrogenase, creatinine iminohydrolase, nicotinamide adenine diphosphate, an alpha-cetoglutaric acid salt, a dilution solution and a hydroxamic acid or a salt thereof.
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